山东医药2017，Vol.57Issue(47)：9-12,4.DOI:10.3969/j.issn.1002-266X.2017.47.003
生长抑素与吉西他滨联合应用对人转移性胰腺癌细胞株AsPC-1增殖的影响
Effect of somatostatin combined with gemcitabine on proliferation of human metastatic pancreatic cancer cell line AsPC-1
摘要
Abstract
Objective To observe the effect of combined therapy with somatostatin (SST) and gemcitabine (Gem) on the proliferation of human metastatic pancreatic cancer AsPC-1 cells.Methods The AsPC-1 cells were cultured in vitro.① The cells were treated with 0,100,200,400,600,and 800 μ,g/mL SST for 24,48,and72 h,then we calculated the IC50 of SST.② The cells were treated with 0,0.1,1 5,10,20,40,and 60 μmol/L Gem for 48 h,and we calculated the IC50 of Gem.After that,AsPC-1 cells were divided into four groups:an untreated control group (group A),a group treated with SST at IC50 (group B),a group treated with Gem at IC50 (group C),and a group treated with SST + Gem at half of their IC50 (group D).At 48 h after treatment,the inhibition rates were calculated.Results For AsPC-1 cells,IC50 of SST was 600 μg/mL at 48 h,and IC50 of Gem was 20 μmol/L.The inhibition rates in the groups A,B,C and D were 0.00％ ± 3.79％,52.59％ ± 4.57％,48.11％ ± 10.93％,and 64.86％ ± 4.83％,respectively.The cell growth was significantly inhibited in the groups B,C,and D as compared with that of group A (P ＜ 0.05),and the inhibition rate was significantly higher in the group D than in the groups B and C (P ＜ 0.05).Conclusion The combination of SST and Gem can inhibit the growth of AsPC-1 cells.关键词
生长抑素/吉西他滨/转移性胰腺癌细胞AsPC-1Key words
somatostatin/gemcitabine/metastatic pancreatic cancer cells AsPC-1分类
医药卫生引用本文复制引用
刘力,饶奇硕,雷昌斌,王文婷,彭钊,旷星星,刘国文..生长抑素与吉西他滨联合应用对人转移性胰腺癌细胞株AsPC-1增殖的影响[J].山东医药,2017,57(47):9-12,4.基金项目
湖南省教育厅资助科研项目(16C1487). （16C1487）
