山东医药2017，Vol.57Issue(47)：20-23,4.DOI:10.3969/j.issn.1002-266X.2017.47.006
溃疡性结肠炎患者TNF-α基因启动子-308G/A单核苷酸多态性观察
Single nucleotide polymorphisms of TNF-α gene promoter-308G/A in patients with ulcerative colitis
摘要
Abstract
Objective To analyze the single nucleotide polymorphisms of tumor necrosis factor-α (TNF-α) gene promoter-308G/A in patients with ulcerative colitis (UC).Methods The single nucleotide polymorphisms of TNF-α gene promoter-308G/A were analyzed in the peripheral blood leukocytes of 750 patients with confirmed UC (observation group) and 750 healthy persons (control group) by polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP).The expression of TNF-α in plasma was determined by ELISA.Results The frequencies of GG,GA,AA genotypes and G,A allele in the observation group were 14.93％,42.27％,42.80％,36.07％,and 63.93％,respectively,and 67.73％,16.40％,15.87％,75.93,and 24.07 in the control group,all P ＜0.05.The levels of TNF-α in the plasma of the observation group and control group were (31.48 ± 11.52) pg/L and (14.82 ± 5.61) pg/L,respectively (P ＜0.05).The levels of TNF-α in patients with GG,GA and AA genotypes of the observation group were (17.95 ±7.82),(34.32 ± 0.75),and (34.64 ± 13.83) pg/L,respectively,while those in the control group were (10.63 ± 3.29) and (21.25 ± 10.47),and (21.91 ± 8.51) pg/L,respectively.The differences were significant between patients with GA and AA genotypes and patients with GG genotype in the same group,and between patients with GG,GA and AA genotypes of the two groups (all P ＜ 0.05).Conclusion There is single nucleotide polymorphism of TNF-α gene promoter-308G/A in patients with UC,and GA and AA genotypes are the risk factors for UC,because they can increase the TNF-α expression in plasma.关键词
肿瘤坏死因子-α基因启动子-308G/A/单核苷酸多态性/溃疡性结肠炎/肿瘤坏死因子αKey words
tumor necrosis factor-α gene promoter-308G/A/single nucleotide polymorphism/ulcerative colitis/tumor necrosis factor-α分类
医药卫生引用本文复制引用
常廷民,张超贤,张利利,李秀敏..溃疡性结肠炎患者TNF-α基因启动子-308G/A单核苷酸多态性观察[J].山东医药,2017,57(47):20-23,4.基金项目
河南省科技厅科技攻关计划(112102310283). （112102310283）
