色谱2018，Vol.36Issue(5)：417-424,8.DOI:10.3724/SP.J.1123.2017.12020
超高效合相色谱-串联质谱法快速测定枸杞中甜菜碱含量
Fast determination of betaine in Lycii Fructus by ultra-performance convergence chromatography-tandem mass spectrometry
摘要
Abstract
Over the past 2500 years,Lycii Fructus has been widely used as a functional food and tonic in Chi-nese herbal medicine. It can nourish the liver and kidneys,moisten the lungs,and improve eyesight. In this study,a new rapid and sensitive method has been developed for the quantitative determination of betaine (index compound)in Lycii Fructus by using ultra-performance convergence chromatography-tandem mass spectrometry(UPC2-MS). The separation of betaine was successfully achieved on an ACQUITY UPC2BEH 2-EP column(150 mm×2.1 mm,1.7 μm),with isocratic elution by a CO2-methanol(80:20,v/v)solvent at a flow rate of 0.7 mL/min. The conditions used in the separation process were as follows:modifier,0.1%(v/v)for-mic acid in methanol;column temperature,40 ℃;backpressure,1.31×107Pa;injection volume,1 μL;and retention time,3 min. The MS system was equipped with an electrospray ionization(ESI)ion source and oper-ated in the selected ion recording(SIR)and positive ion mode. Under the abovementioned conditions,the cali-bration curve was obtained. The linear range of detection was 0.5-50.0 μg/mL,with a correlation coefficient of 0.999 2,and the limit of detection(LOD)was found to be 0.013 μg/mL. The validity of the method was tested by analyses of precision,repeatability,stability,and accuracy(average recovery:96.3%). Finally,the developed method was applied to analyze 11 batches of samples. The results indicated that this method was suitable for evaluating the quality of Lycii Fructus.关键词
超高效合相色谱-串联质谱/甜菜碱/枸杞/质量控制Key words
ultra-performance convergence chromatography-tandem mass spectrometry(UPC2-MS)/betaine/Lycii Fructus/quality control分类
化学化工引用本文复制引用
张弦飞,杨军丽,陈娟,师彦平..超高效合相色谱-串联质谱法快速测定枸杞中甜菜碱含量[J].色谱,2018,36(5):417-424,8.基金项目
National Natural Science Foundation of China(Nos.21775153,21575150) （Nos.21775153,21575150）
the Top Priority Program of"One-Three-Five"Strategic Planning of Lanzhou Institute of Chemical Physics of Chinese Academy of Sciences. （）
