中国生态农业学报2018，Vol.26Issue(6)：884-891,8.DOI:10.13930/j.cnki.cjea.170948
铜绿假单胞菌对镉胁迫水稻苗期生长与镉积累的影响
Effects of Pseudomonas aeruginosa on the growth and cadmium accumulation in rice (Oryza sativa L.) seedling under Cd stress
摘要
Abstract
Heavy metal contamination in rice is a serious problem focused by people all over the world. Microbial passivation is considered as one of effective measures to inhibit heavy metal from environmental into the biological cycle. To investigate the mitigative effects of Pseudomonas aeruginosa on rice seedling exposed to cadmium stress conditions,a set of hydroponic experi-ments by adding 20 μmol·L-1cadmium solution were conducted, and the treatment without added cadmium was used as control (CK). The effects of different treatments on plant growth, cadmium content and cadmium accumulation of rice were determined. The treat-ments included adding with different volume of bacteria suspension, bacterial carrier A (diatomite, size 1-3 mm), carrier B (diatomite, size 3-6 mm), carrier C (activated carbon, specific surface area 1 000 m2·g-1) and microbial inoculum A, B and C, which prepared by carrier A, B and C saturated with bacteria suspension, respectively. The results showed that the growth of root length, plant height and biomass of rice were significantly inhibited by treating with 20 μmol·L-1cadmium. But, the growth of rice was promoted by adding with P.aeruginosa suspension and microbial inoculum A,B and C.In contrast to the treatment only with cadmium(0.523 g·plant-1), total biomass of rice increased by 38.5% to 67.3%, and the highest value was tested in the treatment with inoculum B. Cadmium contents in root, stem-sheath, leaf, as well as in the above parts of rice reduced in all above treatments. Under the treat-ments of microbial inoculum A, B, C and activated carbon, cadmium contents in above parts of rice significantly declined by 45.9%, 47.9%, 59.9% and 59.9%, the transportation factors decreased by 16.7%, 25.0%, 33.3% and 33.3%, as well the bio-concentration factors reduced by 48.1%, 48.8%, 58.8% and 60.9%, respectively. In addition, the cadmium accumulated in rice seedling reduced by 19.2%, 9.5% and 24.3% under the treatments with activated carbon, microbial inoculum A and inoculum C, respectively. While, cadmium accumulation increased by 15.0%, 30.4%, 14.8% and 16.8% under the treatments with microbial inoculum B, bacteria sus-pension A, B and C, which contained 56.4 mL, 45.3 mL and 28.4 mL bacteria, respectively. It meant that the bacteria promote cad-mium accumulation in rice by enlarging biomass of rice. In conclusion, P. aeruginosa exhibited the capacities of promoting rice growth, reducing cadmium content, inhibiting cadmium transportation, as well as cadmium availability of rice under cadmium stress conditions. Higher cadmium passivation abilities of microbial inoculum A, B and C in this study offer us a new promising microbial strain for bio-remediation of environmental cadmium pollution.关键词
铜绿假单胞菌/水稻/镉/载体/菌剂/微生物钝化Key words
Pseudomonas aeruginosa/Rice/Cadmium/Bacteria carrier/Bacteria inoculum/Microbial passivation分类
生物科学引用本文复制引用
何小三,林文雄,王微,肖清铁,郑新宇,郑梅琴,朱静静,韩永明,汪敦飞,林瑞余..铜绿假单胞菌对镉胁迫水稻苗期生长与镉积累的影响[J].中国生态农业学报,2018,26(6):884-891,8.基金项目
科技基础性工作专项(2015YF111300)、福建省自然科学基金项目(2013J01083,2015J01081)及闽台作物特色种质创制与绿色栽培协同创新中心项目[闽教科(2015)75号]资助 This study was supported by the Basic Science and Technology Work of China(2015YF111300),the Natural Science Foundation of Fujian,China(2013J01083,2015J01081)and the Co-innovation Center of Innovative Crop Germplasm Development and Green Cultivation in Fu-jian and Taiwan,China(Project No.75,2015). （2015YF111300）
