生态学报2018，Vol.38Issue(9)：3132-3144,13.DOI:10.5846/stxb201704030572
克隆整合对遮荫白夹竹分株根际土壤细菌生物特征的影响
Effect of clonal integration on soil microbial properties in the rhizosphere of Phyllostachys bissetii, subjected to heterogeneous light
摘要
Abstract
Clonal integration plays an important role for clonal plants during adaptation to heterogeneous habitats,by enhancing the survival of clonal fragments,thus,improving growth performance.An in-situ experiment was conducted using a clonal fragment of Phyllostachys bissetii with two successive ramets.We focused on microbial properties in the rhizosphere soil of shaded,distal,or proximal ramet.Shading (80％ shading) was applied to distal or proximal ramets,respectively,whereas counterparts were placed in full sunlight.The rhizome between two successive ramets was either severed or retained intact.In present study,dissolved organic carbon (DOC),microbial biomass,and two extracellular enzymes (N-acetyl-β-D-glucosaminidase,urease) were investigated in the rhizosphere soil of shaded distal and proximal ramets.The composition of the soil microbial community in the rhizosphere of shaded distal and proximal ramets was confirmed.The key results of this study are as follows:DOC in the rhizosphere soil of shaded distal or proximal ramets was significantly increased by clonal integration.Furthermore,increased microbial biomass carbon and nitrogen (MBC,MBN) were observed in the rhizosphere soil of shaded distal and proximal ramets.Clonal integration significantly enhanced the activities of N-acetyl-β-D-glucosaminidase (NAGase) and urease in the rhizosphere soil of shaded distal and proximal ramets.A total of 556519 reads and 18898 OTUs were obtained from the 12 soil samples through Illumina Miseq sequencing.The bacterial OTUs were assigned into 13 different phyla and 415 genera.Nitrosomonadaceae (uncultured),Nitrospira,Nitrospinaceae (uncultured),Xanthobacteraceae(uncultured),Bradyrhizobium were associated with soil N cycling,with low relative abundance.The results of principal component analysis revealed that the effect of clonal integration on the composition of the soil microbial community in the rhizosphere of shaded distal and proximal ramets were not significant,sharing similar microbial diversity at the OTU level.In summary,C availability and soil microbial biomass were significantly increased by clonal integration in the rhizosphere soil of shaded distal and proximal ramets and further stimulated the turnover of soil organic matter mediated by soil microbes in the rhizosphere.We tentatively conclude that the effect of clonal integration on soil microbial properties in the rhizosphere soil may partly explain the important ecological advantage of clonal plants,especially the species subjected to heterogeneous light habitats.关键词
克隆整合/根际土壤/高通量测序/细菌生物群落结构/功能冗余Key words
clonal integration/rhizosphere soil/Illumina Miseq sequence/microbial community composition/functional redundancy引用本文复制引用
薛阁,李洋,陈劲松,宋会兴..克隆整合对遮荫白夹竹分株根际土壤细菌生物特征的影响[J].生态学报,2018,38(9):3132-3144,13.基金项目
国家自然科学基金项目(31470636) （31470636）
长江上游生态安全协同创新中心开放基金项目 （）
