生态学报2018，Vol.38Issue(9)：3347-3356,10.DOI:10.5846/stxb201706151092
高通量测序技术在野生动物食性分析中的应用
The application of high-throughput sequencing technologies to wildlife diet analysis
摘要
Abstract
Diet related studies are critical in animal ecology,and diet analysis methods have been continuously improved and updated due to technical and application limitations of the different methods.The high-throughput sequencing (HTS) technique is being used to investigate the diets of wildlife.This use has greatly improved the efficiency and broadened the range of its application to diet analysis.HTS has obvious advantages of improved dataset analysis,sensitivity,and resolution,but HTS-based diet analysis is still a weak research field because it involves many different steps and is influenced by complex factors.In this study,we summarize the general strategies adopted for diet analysis using HTS,review the current progress of this technique in analyzing diet components,and elucidate the intra-specific and inter-specific dietary relationship and the relationship between food resources and habitat and behavior.Then the influences of PCR bias,contamination,and quantitative analysis on accurate diet analysis are discussed.Ways to improve the technique are proposed,as are the prospects for the future application of this method.关键词
高通量测序/粪便DNA/食性分析/种内关系/种间关系/觅食行为Key words
high-throughput sequencing/fecal DNA/diet analysis/inter-specific relationship/intra-specific relationship/foraging behavior引用本文复制引用
刘刚,宁宇,夏晓飞,龚明昊..高通量测序技术在野生动物食性分析中的应用[J].生态学报,2018,38(9):3347-3356,10.基金项目
中央级公益性科研院所基本科研业务费专项资金(CAFINT2015K05) （CAFINT2015K05）
国家自然科学基金(31500303) （31500303）
