生物技术通报2018，Vol.34Issue(3)：53-58,6.DOI:10.13560/j.cnki.biotech.bull.1985.2017-0756
数字PCR在转基因水稻拷贝数鉴定中的应用
Estimation of the Copy Number of Exogenous Genes in Genetically Modified Rice by Droplet Digital PCR
摘要
Abstract
The varied segments from field-growing 1-year old Pinus serotina spring stem and shoot tips from seed germination were used as explants.The explants were disinfected by 0.1％ HgCl2, and those with favorable disinfection were used as sterile materials, and then the combination of medium type and the hormones for the axillary bud induction were optimized.The results showed that 10 min disinfection of middle parts of the spring stems by 0.1％ HgCl2 was the optimal, the pollution rate was the lowest (0.8), and the best combination of medium and hormone levels to induce axillary buds was MS medium with 2.5 mg/L 6-BA and 0.2 mg/L NAA.And the optimal disinfected time of seed was 4 min, the pollution rate was 2.4％, and the induction rate was the highest in the medium containing 0.7 mg/L NAA and 1.0 mg/L 6-BA. In summary, an effective method to induce the axillary buds from the spring stem and seeds is established, which may provide theoretical evidences and technical support for the rapid and efficient breeding of Pinus serotina.关键词
转基因水稻/目的基因拷贝数/微流滴数字PCRKey words
Pinus serotina/disinfection/spring stem segment/axillary bud/tissue culture引用本文复制引用
王永,兰青阔,赵新,陈锐,沈晓玲,李文,张耀中,李亮,王勤英..数字PCR在转基因水稻拷贝数鉴定中的应用[J].生物技术通报,2018,34(3):53-58,6.基金项目
国家自然科学基金面上项目(31671964),转基因重大专项(2016ZX08012003) （31671964）
