生物技术通报2018，Vol.34Issue(3)：225-229,5.DOI:10.13560/j.cnki.biotech.bull.1985.2017-0820
葡萄籽中白藜芦醇的提取及其对LO2细胞氧化损伤保护及延缓衰老作用
Resveratrol Extraction from Grape Seed and Its Effect on LO2 Cell Oxidative Damage Protection and Anti-aging
摘要
Abstract
Resveratrol was extracted from grape seed by ultrasonic assisted two-phase aqueous method.The oxidative damage model was constructed by inducing LO2 cell with alcohol(100 mmol/L),and aging mice model was built via D-galactose.The superoxide dismutase(SOD),glutathione peroxidase (GSH-px) activities and malondialdehyde (MDA) content in the cells and mice liver homogenate were measured after intervention by resveratrol extracts.Resveratrol extraction yield was 2.408 ± 0.088 mg/g when liquid ratio was 1 ∶ 10,leaching time was 40 min,ultrasonic time,power and temperature were 300 W,10 min and 50℃,and aqueous two-phase system was ethanol ∶ ammonium sulphate =1 ∶ 1.Resveratrol extracts affecting LO2 cell reduced cells MDA content and increased the activity of SOD and GSH-px induced by ethanol.Moreover,resveratrol extracts presented a certain inhibitory effects on liver MDA production,and increased the activities of SOD and GSH-px in aging model mice.关键词
葡萄籽/白藜芦醇/LO2细胞/氧化损伤Key words
grape seed/resveratrol/LO2 cells/oxidative damage引用本文复制引用
高原,王福玲,徐蓓蕾,雒江菡,阎力君,赵莹莹,王越..葡萄籽中白藜芦醇的提取及其对LO2细胞氧化损伤保护及延缓衰老作用[J].生物技术通报,2018,34(3):225-229,5.基金项目
国家自然科学基金面上项目(31370764),哈尔滨市科技局青年后备人才项目(2016RQQXJ115),黑龙江省普通高校微生物重点实验室(黑龙江大学)开放基金项目(2012MOI-6) （31370764）
