山西农业大学学报（自然科学版）2018，Vol.38Issue(5)：1-6,6.DOI:10.13842/j.cnki.issn1671-8151.201712009
一株百合枯萎病菌的鉴定及其生物学特性研究
Identification and biological characteristics of a fungal isolate causing lily Fusarium wilt
摘要
Abstract
[Objective]The purpose of the study was to clarify the pathogen causing lily wilt disease in Taigu and to de-termine its biological characteristics.[Methods]The pathogen was isolated and identified by tissue isolation,pathoge-nicity test,morphological characteristics study,and rDNA -ITS sequence analysis.Biological characteristics of the pathogen were determined by measuring the diameter of the colony and counting the number of conidia using hemocy-tometer measurement.[Results]The pathogen which caused lily wilt disease was identified as Fusarium proliferatum. The optimal temperature for mycelial growth and sporulation was between 25 and 30 ℃,while the optimal pH value for mycelial growth and sporulation were at 7,and 6-7,respectively.Saccharose and lactobiose were the best carbon sources for mycelial growth and peptone was the best nitrogen source.The best carbon source for sporulation was sac-charose and the best nitrogen source was glycine.[Conclusion]The pathogen of lily wilt disease was F.proliferatum. The understanding of its primary biological property provided the theoretical basis for disease control.关键词
百合枯萎病/病原菌鉴定/层出镰刀菌/生物学特性Key words
Lily wilt/Pathogen identification/Fusarium proliferatum/Biological characteristics分类
农业科技引用本文复制引用
翟雅鑫,姚晨阳,薛丽芳,曹挥,李新凤,杜方,郝晓娟..一株百合枯萎病菌的鉴定及其生物学特性研究[J].山西农业大学学报（自然科学版）,2018,38(5):1-6,6.基金项目
山西省应用基础研究项目(K271704003) （K271704003）
