山西农业大学学报（自然科学版）2018，Vol.38Issue(5)：23-32,39,11.DOI:10.13842/j.cnki.issn1671-8151.201803008
葡萄MYB基因家族及其对花器官性别分化调控的分析
Analysis of grape MYB gene family and its regulation on sex differentiation of flower organs
摘要
Abstract
[Objective]Present study was conducted to better understanding the MYB gene family and to explore the re-lationship between MYB gene family and sex differentiation of grape floral organs which would provide evidence for fur-ther research on sex differentiation of floral organs in grape.[Methods]Bisexual flowers of 'Beichun'grape species at full-blooming stage(corolla was off),single-male flowers of '520A'grape species,and female flowers of'1316C'grape species were selected as testing materials.Three repelications were set up.RNA was extracted from each sample to an-alyze transcriptome sequencing,and bioinformatics analysis.[Results]Four genes were expressed in the genomes of different sex flower organs,and the expression of the V IT_14s0066g01090 was the highest.V IT_13s0067g01630 and V IT_12s0059g00700 expression in single male flowers were significantly higher than that in bisexual flowers and '1316C'female flowers,while no significant difference of gene expression was detected between bisexual flowers and fe-male flowers.The expression level of V IT_19s0015g01280 was the highest in female flowers and significantly higher than that in bisexual flower,however there was no significant difference of the expression of V IT_19s0015g01280 be-tween female flowers and single male flowers.The phylogenetic tree showed that VIT_19s0015g01280 and AtMYB80 were clustered into one branch.[Conclusion]It was preliminarily determined that some genes from MYB gene family were involved in regulating sex differentiation of grape floral organs,which were the candidate genes to control sex dif-ferentiation of floral organs.The present study provided a theoretical reference for further research on sex differentia-tion of floral organs in grape.关键词
葡萄/转录因子MYB/性别分化/基因功能Key words
Grape/MYB transcription factors/Sexual differentiation/Gene function分类
农业科技引用本文复制引用
焦晓博,罗尧幸,赵伟,刘榕晨,李雪雪,纪薇..葡萄MYB基因家族及其对花器官性别分化调控的分析[J].山西农业大学学报（自然科学版）,2018,38(5):23-32,39,11.基金项目
国家自然科学基金(31401834) （31401834）
山西省高等学校科技创新项目(2016153) （2016153）
山西农业大学青年拔尖创新人才支持计划(TYIT201401) （TYIT201401）
山西省科技重点研发项目(201603D211105-9、201703D221014-4) （201603D211105-9、201703D221014-4）
山西省研究生教育创新项目(2017SY034) （2017SY034）
山西省高等学校优秀青年学术带头人(2017) （2017）
