天津农业科学2018，Vol.24Issue(5)：1-4,4.DOI:10.3969/j.issn.1006-6500.2018.05.001
恶臭假单胞菌UW4中趋化受体蛋白的克隆、表达及鉴定
Cloning,Expression and Identifacation of Chemotaxis Receptor Protein in Pesudomonas UW4
李涛1
作者信息
- 1. 河南农业大学农业部农业微生物酶工程重点实验室,河南郑州450002;三门峡职业技术学院食品园林学院,河南三门峡472000
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摘要
Abstract
The plant growth-promoting rhizosphere bacteria became more and more striking because it could promote the growth, pre-vent and treat diseases of plant.These researches mainly concentrated on the Pesudomonas.Pesudomonas colonization in plant rhizo-sphere was related to chemotaxis.This study screened the methyl chemotaxis receptor proteins(MCPs)of Pesudomonas UW4 by using several bioinformatics software,and then cloned and expressed the MCPs.The result showed that the cloned MCP1 could express sta-bly, through the purification of the methyl chemotaxis receptor proteins of Pseudomonas UW4 and analysis the thermodynamic effect of the different ligands with MCPs,we could find out the specificity chemotaxis receptors of Pesudomonas UW4,clarify its chemotaxis mechanism and give some theoretical supporting to the growth-promoting of plant.关键词
植物根际促生菌/趋化/ACC脱氨酶/MCP/SDS-PAGEKey words
plant growth-promoting rhizosphere bacteria/chemotaxis/ACC deaminase/methyl chemotaxis receptor protein/SDS-PAGE分类
生物科学引用本文复制引用
李涛..恶臭假单胞菌UW4中趋化受体蛋白的克隆、表达及鉴定[J].天津农业科学,2018,24(5):1-4,4.
