畜牧兽医学报2018，Vol.49Issue(5)：897-906,10.DOI:10.11843/j.issn.0366-6964.2018.05.004
TLR5基因启动子区甲基化修饰与苏太断奶仔猪E.coli F18抗性的关系
Relationship between Methylation in Promoter Region of TLR5 Gene and Resistance to Escherichia coli F18 of Sutai Weaned Piglets
摘要
Abstract
The aim of this study was to investigate the regulation function of methylation in promoter region of TLR5 gene on the resistance to E.coli F18 of weaned piglets.The differential expressions of TLR5 gene and protein in small intestine (duodenum and jejunum) between E.coli F18-resistant and E.coli F18-sensitive Sutai weaned piglets were detected by qPCR and Western blot,respectively.The core promoter region,CpG islands and their acting elements of TLR5 were determined by bioinformatics analysis and dual luciferase reporter system,and the correlation between the methylation degree in promoter region and the expression of TLR5 gene in E.coli F18-resistant and E.coli F18-sensitive Sutai weaned piglets was detected and analyzed.The results showed that expression levels of TLR5 gene in duodenum and jejunum of sensitive individuals were significantly and very significantly higher than that in resistant individuals (P＜0.05 and P＜0.01),respectively.And the protein expressions of TLR5 in duodenum and jejunum of sensitive individuals were significantly higher than that of resistant individuals (P＜0.05).The core promoter region of TLR5 gene included 2 CpG islands and 16 acting elements,the methylation level of the mC 6 site in the second CpG island of the promoter region had a certain regulation effect on the expression of TLR5 gene.The site located in the core promoter bound by transcrip tion factor Spl.The above results indicated that TLR5 gene played an important regulating role in the process of E.coli invasion,whose low expression was conducive to the piglets' resistance to E.coli.The methylation of the mC-6 CG site in the second CpG island of the TLR5 gene promoter could inhibit the binding of the transcription factor Spl,which inhibited the expression of the TLR5 gene and ultimately affected the resistance of weaned piglets to E.coli.关键词
苏太猪/TLR5基因/甲基化/大肠杆菌F18/相关性分析Key words
Sutai pig/TLR5 gene/methylation/Escherichia coli F18/correlation analysis分类
农业科技引用本文复制引用
戴超辉,冯海悦,宗秋芳,吴圣龙,包文斌..TLR5基因启动子区甲基化修饰与苏太断奶仔猪E.coli F18抗性的关系[J].畜牧兽医学报,2018,49(5):897-906,10.基金项目
国家自然科学基金(31772560 （）
31572360) （）
江苏省科技支撑计划(BE2016315) （BE2016315）
扬州大学中青年学术带头人资助项目 （）
