畜牧兽医学报2018，Vol.49Issue(5)：954-961,8.DOI:10.11843/j.issn.0366-6964.2018.05.010
LEPR及LEP基因表达量对FecB不同基因型小尾寒羊产羔数的影响
Effects of Expression of LEPR and LEP Genes on Litter Size of Small Tail Han Sheep with Different FecB Genotypes
摘要
Abstract
The aim of this study was to determine if the expression of leptin receptor (LEPR) and leptin (LEP) genes was associated with litter size of Small Tail Han ewes.Twenty one healthy Small Tail Han ewes,composed of 9 FecB wild type (++) and 12 FecB mutation homozygous type (BB),about 2-3 years old,were used to investigate the ovulation rate by laparoscopy after spontaneous estrus with CIDR.Real-time PCR was used to detect the expression of LEPR and LEP genes in 11 tissues (heart,liver,spleen,lung,kidney,adrenal gland,fallopian tube,uterus,hypothalamus,pituitary and ovary)of Small Tail Han ewes during follicular phase and in hypothalamus-pituitary-ovary gonadal axis of Small Tail Han ewes (+ + and BB genotypes) during luteal phase and follicular phase.The results showed that both ovulation number (P=0.003 5) and litter size (P=0.004 0) of individuals with BB genotype were significantly higher than that of individuals with + + genotype.These 2 genes were all expressed in 11 tissues during follicular phase,the expression of LEPR gene was high in ovary,uterus and adrenal gland,and the expression of LEP gene was high in hypothalamus,pituitary,ovary and kidney.In ovary,the expression of LEPR gene of individuals with + + genotype during follicular phase was higher (P＞0.05) than that during luteal phase.The LEPR expression of individuals with BB genotype during follicular phase was significantly higher than that of individuals with + + genotype during follicular phase (P=0.007),BB genotype during luteal phase (P=0.003) and + + genotype during luteal phase (P =0.003).Therefore,we concluded that there was a positive correlation between the high expression of LEPR gene during follicular phase and high litter size in Small Tail Han ewes,which provided a new clue for improving litter size in Small Tail Han sheep.关键词
小尾寒羊/FecB/产羔数/瘦素基因/瘦素受体基因Key words
Small Tail Han sheep/FecB/litter size/LEP/LEPR分类
农业科技引用本文复制引用
于嘉瑞,张效生,张金龙,孙伟,储明星,王翔宇,郭晓飞,贺小云,孙庆,文禹粱,狄冉,刘秋月,胡文萍..LEPR及LEP基因表达量对FecB不同基因型小尾寒羊产羔数的影响[J].畜牧兽医学报,2018,49(5):954-961,8.基金项目
国家自然科学基金(31772580) （31772580）
国家转基因科技重大专项(2016ZX08009-003-006 （）
2016ZX08010-005-003) （）
国家肉羊产业技术体系专项(CARS-38) （CARS-38）
中央级公益性科研院所基本科研业务费专项(Y2017JC24 （）
2017ywf-zd-13) （）
中国农业科学院科技创新工程(ASTIP-IAS13 （）
CAAS-XTCX2016010-01-03 （）
CAAS-XTCX2016010-03-03 （）
CAAS-XTCX2016011-02-02) （）
宁夏农林科学院科技创新先导资金(DWJLC-2016001) （DWJLC-2016001）
内蒙古自治区科技重大专项 （）
农业科研杰出人才及其创新团队项目 （）
国家万人计划科技创新领军人才项目 （）
天津市科技计划项目(16ZXZYNC00050) （16ZXZYNC00050）
