畜牧兽医学报2018，Vol.49Issue(5)：962-970,9.DOI:10.11843/j.issn.0366-6964.2018.05.011
屎肠球菌对大肠杆菌感染肉鸡生长性能、血清生化指标和盲肠菌群结构的影响
Effects of Enterococcus faecium on the Growth Performance,Serum Biochemical Parameters and the Structure of Cecal Microflora in the E.coli-challenged Broilers
摘要
Abstract
The present study was conducted to investigate the effects of Enterococcus faecium administration on the growth performance,serum biochemical parameters and cecal microflora's structure in E.coli-challenged broilers.A total of 360 1-day-old White Feather chickens with the approximative body weight were randomly divided into 4 groups,six replicates (15 birds per replicate) in each group:negative control (NC),positive control (basal diet,PC),E.faecium-fed (E.f) and antibiotic-fed birds (Anti).The birds in PC,E.f and Anti groups were challenged with E.coli K88 on d 7,the whole test lasted for 28 d.And the serum levels of complement components and immunoglobulins were measured by the specific kits,while the change of cecal microbiota was determined by PCR-DGGE.Data showed that..1) The birds in E.f group had significant higher body weight than that of birds in NC and PC groups on d 14,21 and 28(P＜0.05);The birds in E.f group had significant higher ADG than that of birds in NC and PC groups during d 10-14,15-21 and 10-28 (P＜0.05).2) The birds in E.f and Anti groups had significant higher level of serum IgA than that of birds in NC and PC groups on d 14,21 (P＜0.05);Birds in E.f and Anti groups had significant higher level of serum IgG than that of birds in NC group in d 10-28(P＜0.05).3) Compared with birds in PC group,birds in E.f group had significant higher C3 concentration on d 21(P＜0.05).The birds in PC,E.f and Anti groups had higher serum lysozyme than that of birds in NC group on d 10,14 and 21 (P＜0.05).4) Compared with the birds in PC group,birds in E.f group had significant higher Shannon index of cecal microflora (P＜0.05) on d 28.In conclusion,the dietary Enterococcus faecium improved the growth performance,serum immune parameters and changed the diversity of cecal intestinal microflora in the E.coli K88-challenged chicken.关键词
屎肠球菌/生长性能/血液生化指标/肠道菌群/大肠杆菌K88/肉鸡Key words
Enterococcus faecium/growth performance/serum biochemical parameters/intestinal microflora/E.coli K88/broilers分类
农业科技引用本文复制引用
曹广添,代兵,张玲玲,曾新福,杨彩梅..屎肠球菌对大肠杆菌感染肉鸡生长性能、血清生化指标和盲肠菌群结构的影响[J].畜牧兽医学报,2018,49(5):962-970,9.基金项目
浙江省重点研发计划项目(2017C02005) （2017C02005）
浙江省“饲料研发与安全科技创新团队”项目(2011R50025) （2011R50025）
