畜牧兽医学报2018，Vol.49Issue(5)：1005-1011,7.DOI:10.11843/j.issn.0366-6964.2018.05.015
gga-miR-140-3p抑制马立克病病毒转化细胞MSB1增殖、迁移和侵袭
gga-miR-140-3p Inhibit Proliferation, Migration and Invasion in MDV-transformed MSB1 Cells
摘要
Abstract
Specific pathogen free Leghorn chickens were infected with MDV,and lymphoma from spleens and livers were collected post infection.The qRT-PCR assay showed that microRNA ggamiR-140-3p was downregulated in MDV-induced tumorous spleen and lymphoma from liver.To elucidate the functions of this miRNA in Marek's disease tumor transformation,we transfected MDV-transformed lymphoid cell line,MDCC-MSB1 with gga-miR-140-3p agomir or agomir NC,and inspected their effects on cell proliferation and migration as well as MMP2 and MMP9 genes expression levels whose expression levels were closely correlated with invasion.The proliferation of MSB1 cells decreased at 24,36,48,60 and 72 h post gga-miR 140 3p agomir transfection.The cell number of migration also decreased after transfection with gga-miR-140-3p agomir.MMP2 expression was significantly downregulated at 24,48 and 72 h;in addition,MMP9 transcription was remarkably downregulated at 48 h post gga-miR-140-3p agomir transfection.In conclusion,gga-miR-140-3p which was downregulated in MDV-induced tumorous tissues had an effect on features of tumor cells such as proliferation,migration and invasion,which indicates that this miRNA may be involved in Marek's disease tumor transformation.关键词
马立克病病毒/增殖/迁移/MMP2基因/MMP9基因Key words
Marek's disease virus/proliferation/migration/MMP2 gene/MMP9 gene分类
农业科技引用本文复制引用
赵春芳,李新,韩博,曲鲁江,刘长军,Jiuzhou Song,连玲,杨宁..gga-miR-140-3p抑制马立克病病毒转化细胞MSB1增殖、迁移和侵袭[J].畜牧兽医学报,2018,49(5):1005-1011,7.基金项目
863计划(2013AA102501) （2013AA102501）
国家自然科学基金(31320103905 （）
31301957) （）
教育部长江学者及创新团队发展计划(IRT_15R62) （IRT_15R62）
现代农业产业技术体系(CARS-41) （CARS-41）
青年科学家托举项目 （）
畜禽种质资源平台和青年教师创新计划 （）
