畜牧兽医学报2018，Vol.49Issue(5)：1012-1019,8.DOI:10.11843/j.issn.0366-6964.2018.05.016
基于线粒体16S rRNA全序列分析我国绵羊痒螨兔亚种的遗传多样性
Genetic Diversity of Psoroptes ovis var.cuniculi in China,Basing on the Complete Sequence of Mitochondrial 16S rRNA
摘要
Abstract
In order to evaluate the genetic variation and population structure of Psoroptes ovis var.cuniculi,the full-length of the mitochondrial 16S rRNA of 83 isolates of Psoroptes ovis var.cuniculi from 5 geographic regions in China (including North,East,central,southwest,northwest zones) were amplified using the PCR technology,sequenced and then genetically analyzed.The results showed that the full 16S rRNA gene from 83 Psoroptes ovis var.cuniculi isolates were 1 025 bp in length,and that there were a total of 83 polymorphic sites and 52 haplotypes;the values of Fst and the gene flow between 5 geographical populations were-0.037 59-0.587 63 and -6.900 7-31.117 62,respectively;NJ tree and haplotype network analysis further showed these 52 haplotypes formed two branches with a messy distribution,and no population cluster patterns according to the geographical area,temperature zone and rabbit breeds were observed.These results indicate that Psoroptes ovis var.cuniculi population in China appeared a high genetic diversity and differentiation,but the geographical population structure has not formed.关键词
绵羊痒螨兔亚种/16S rRNA基因/遗传多样性Key words
Psoroptes ovis var.cuniculi/16S rRNA gene/genetic diversity分类
农业科技引用本文复制引用
古江,李燕芳,赵习彬,谢跃,赖为民,杨光友,古小彬..基于线粒体16S rRNA全序列分析我国绵羊痒螨兔亚种的遗传多样性[J].畜牧兽医学报,2018,49(5):1012-1019,8.基金项目
国家自然科学基金青年基金(31402188) （31402188）
四川农业大学学科“双支计划”(SC-03570422) （SC-03570422）
