畜牧兽医学报2018，Vol.49Issue(5)：1020-1026,7.DOI:10.11843/j.issn.0366-6964.2018.05.017
马流产沙门菌鞭毛蛋白FliC对马腺疫链球菌SeM蛋白免疫效应分析
Immune Efficacy Analysis of Flagellin Protein FliC of Salmonella abortus equi on SeM of Streptococcus equi subsp, equi
摘要
Abstract
The aim of this study was to investigate the effects of the flagellin protein FliC of Salmonella abortus equi on immune response of Streptococcus equi subsp.equi SeM and provide basis for the development of a new effective strangle vaccine.The recombinant protein FliC was constructed,expressed and purified using affinity chromatography,and its immunogenicity was analyzed by Western blot.The purified FliC and SeM were used in-combination to immunize C57BL/6 mice and the serum antibody level,antibody subtype of immunized mice and challenge protection rate were investigated.SDS-PAGE result indicated that the expressed recombinant FIiC protein was 52 kDa.The results of antibody typing showed that the main antibodies were mainly IgG1,and the challenge protection rate of FliC + SeM immunization group (87％) was higher than that of SeM immunization group (67 ％).The flagellin FliC of Salmonella abortus equi could enhance the immune efficacy of SeM and this result laid experimental foundation for the further utilization of SeM.关键词
马流产沙门菌/FliC/马腺疫链球菌/SeM/免疫效应/免疫保护力Key words
Salmonella abortus equi/FliC/Streptococcus equi subsp.equi/SeM/immune efficacy/immune protection分类
农业科技引用本文复制引用
赵亚南,杨康,张宝江,李斌,苏艳..马流产沙门菌鞭毛蛋白FliC对马腺疫链球菌SeM蛋白免疫效应分析[J].畜牧兽医学报,2018,49(5):1020-1026,7.基金项目
国家自然科学基金(31660707) （31660707）
新疆维吾尔自治区重大科技专项项目(2017A01002-2) （2017A01002-2）
新疆维吾尔自治区青年科技人才培养项目(qn2015yx013) （qn2015yx013）
