畜牧兽医学报2018，Vol.49Issue(5)：1027-1036,10.DOI:10.11843/j.issn.0366-6964.2018.05.018
中药复方芩藿饮对鸭肝炎病毒实验感染雏鸭的治疗效果分析
Analysis of the Therapeutic Effect of Traditional Chinese Herbal Compound of Qinhuo Oral Liquid in Ducklings Infected with Duck Hepatitis Virus
摘要
Abstract
The purpose of this study was to evaluate the therapeutic effect of traditional Chinese herbal compound of Qinhuo oral liquid on ducklings that infected with duck hepatitis virus.Three hundred feathers healthy 1-day-old ducklings were randomly divided into 5 groups,60 feathers in each group.The test groups were Qinhuo high dose group,middle dose group,low dose group,virus group and blank control group,respectively.At the age of 6 days old,except for the blank control group,the other groups were intramuscular injected with DHAV-1 to establish artificial infection model;1 h later,Qinhuo high dose group,middle dose group and low dose group were given high dose (0.6 mL per feather),middle dose (0.4 mL per feather) and low dose (0.2 mL per feather) of Qinhuo oral liquid in the water for treatment,once a day for 3 days,the virus group and blank control group treated without medicine.The samples were collected at the 4th,8th and 48th hour during the experiment for measuring related indexes.During the experiment,the mortality rate of each group was compared;the liver pathological examination and HE staining sec tion were observed;relative blood DHAV 1 VP1 gene copies number was analyzed;the contents of hepatic function biochemical indexes (AST,ALT,TP,ALB,GLO) and serum cytokines (IL 2,IL-6,IL-8,IFN-β) were detected.All indexes were severed to evaluate the therapeutic effect of Qinhuo oral liquid on duck virus hepatitis.The results showed that Qinhuo oral liquid can effectively reduce the mortality rate of infected ducklings;compared with virus group,Qinhuo oral liquid can significantly reduce the copies number of DHAV 1 VP1 in the blood (P＜0.05);repair the liver damage casused by DHAV-1;reduce the contents of AST and ALT;enhance the contents of IL-2,IL-6,IL-8,IFN-β in serum.Considering all factors,the high dose of the Qinhuo oral liquid has a better therapeutic effect on ducklings infected with duck hepatitis virus.关键词
中药复方/鸭病毒性肝炎/芩藿饮/治疗效果Key words
Chinese herbal medicine compound/duck virus hepatitis/Qinhuo oral liquid/therapeutic effect分类
农业科技引用本文复制引用
张伟,白景英,杜红旭,熊文,明珂,刘家国..中药复方芩藿饮对鸭肝炎病毒实验感染雏鸭的治疗效果分析[J].畜牧兽医学报,2018,49(5):1027-1036,10.基金项目
中央高校基本科研业务费(Y0201700441) （Y0201700441）
国家自然科学基金(31572557 （）
31772784) （）
公益性行业(农业)科研专项课题(201303040 （农业）
201403051) （）
