畜牧兽医学报2018，Vol.49Issue(5)：1037-1046,10.DOI:10.11843/j.issn.0366-6964.2018.05.019
槲皮素通过影响Nrf2-Keap1信号通路减缓镉中毒大鼠的生殖机能损伤
Protective Effects of Quercetin on the Reproductive Injury Induced by Cadmium through the Nrf2-Keap1 Signaling Pathway in Rats
摘要
Abstract
The aim of this study was to investigate the effect of Quercetin (QE) on the reproductive system damage induced by cadmium (Cd) in male rats and the role of Nrf2-Keapl signaling pathway in this process.A total 32 3-month-old SPF Wistar male rats were randomly divided into four groups with eight rats in each group:control group [0.5 mL · (kg · d)-1 physiological saline],Cd group [5 mg · (kg · d)-1 CdCl2],QE group [15 mg · (kg · d)-1 QE],Cd+ QE group [5 mg· (kg · d)- 1 CdCl2+15 mg · (kg · d)-1 QE],the study lasted for 30 days.At the end of the trial,the testis and epididymis organs coefficients were calculated,then the sperm quality and the levels of anti-oxidation of testis were measured.RT-qPCR and Western blot were used to detect the changes of mRNA and protein expressions of Nrf2 and its related genes,respectively.The results showed that the reproductive function of rats in Cd group was seriously damaged compared with the control group.Compared with Cd group,the coefficients of testis and epididymis (P＜0.01) and the secretion testosterone of rats in Cd+QE group were signifi cantlyincreased (P＜0.05).The semen quality was improved,furthermore,the levels of MDA,H2O2 were significantly decreased (P＜0.01) and the levels of GSH (P＜0.05),CAT,T-SOD,GSH-Px (P＜0.01) were significantly increased in Cd+QE group.The analysis showed that the mRNA and protein expression levels of Nrf2,HO 1,NQO1,GSH-Px,γ-GCS in testis were sig nificantly increased (P＜0.05 or P ＜0.01) and Keapl mRNA and protein expression levels were significantly decreased (P ＜0.05) in Cd+QE group.In conclusion,QE can effectively alleviate the reproductive system damage of male rats induced by Cd through activating Nrf2-Keapl signaling pathway.关键词
槲皮素/镉/睾丸/氧化应激/Nrf2-Keap1信号通路Key words
Quercetin/cadmium/testis/oxidative stress/Nrf2-Keapl signaling pathway分类
农业科技引用本文复制引用
何刘,李慧,周厚继,佟翠,王佳美,李美琦,杨淑华,何剑斌..槲皮素通过影响Nrf2-Keap1信号通路减缓镉中毒大鼠的生殖机能损伤[J].畜牧兽医学报,2018,49(5):1037-1046,10.基金项目
沈阳农业大学博士后基金(2014.6) （2014.6）
