畜牧兽医学报2018，Vol.49Issue(5)：1054-1060,7.DOI:10.11843/j.issn.0366-6964.2018.05.021
不同时间异氟醚麻醉对幼龄大鼠海马神经元凋亡及自噬的影响
Effects of Different Isoflurane Anesthesia Time on Neural Apoptosis and Autophagy in the Developing Rat Hippocampus
摘要
Abstract
Studies have shown that isoflurane could cause autotrophy and apoptosis in the develo ping rat brain.Based on the preliminary experiment,forty 7 day Sprague-Dawley rat pups were selected and divided into control group and groups of 1.5 ％ isoflurane anesthesia for 2,3,4 and 6 h randomly.After anesthesia,the rats were euthanized,then hippocampus were collected for TUNEL-staining,LC3-Ⅱ immunohistochemistry and Western blotting,to investigate the level of autophagy and apoptosis.The results showed that with the increasing time exposed to isoflurane,the percent of positive cells were significantly increased at 3 h,the results of LC3-Ⅱ immunohistochemistry showed that the percent of positive cells were increased at 3 and 4 h,but decreased at 6 h;and the expression of Caspase-3 was increased gradually compared with control group,although the expression of Bcl 2 was decreased.However,the expression of Beclin-1 and LC3-Ⅱ/I were increased at the first 4 h with the most expressions at 3 h (P＜0.05) and then decreased;In addition,compared with isoflurane anesthesia at 3 and 4 h,the expression amount of Beclin 1 was significantly decreased at 6 h while Caspase-3 significantly increased (P＜0.05).These results showed that isoflurane can not only induce extensive apoptosis of hippocampal neurons in young SD rats,but also induce autophagy in hippocampal neurons.And the level of apoptosis gradually increased with the prolongation of anesthetic time,while the level of autophagy increased first and then decreased.Autophagy may play a protective role in the apoptosis of hippocampal neurons induced by isoflurane.关键词
全身麻醉/异氟醚/自噬/凋亡/幼龄大鼠Key words
general anesthesia/isoflurane/autophagy/apoptosis/developing rat分类
农业科技引用本文复制引用
邱子豪,胡学远,冯秀晶,赵元,宋曼玉,王超然,刘涛,范宏刚..不同时间异氟醚麻醉对幼龄大鼠海马神经元凋亡及自噬的影响[J].畜牧兽医学报,2018,49(5):1054-1060,7.基金项目
国家自然科学基金(31772806) （31772806）
围家重点研发计划子课题(2016YFD051008) （2016YFD051008）
黑龙江省自然科学基金(C2017022) （C2017022）
东北农业大学大学生创新训练项目(201710224062) （201710224062）
