畜牧兽医学报2018，Vol.49Issue(5)：1081-1088,8.DOI:10.11843/j.issn.0366-6964.2018.05.024
三黄种鸡场孵化死胚中沙门菌的分离鉴定与耐药性分析
Isolation, Identification and Antimicrobial Resistance of Salmonella from Dead Chicken Embryos in Sanhuang Breeder Farms
摘要
Abstract
To investigate current infection statuses of Salmonella in dead chicken embryos,Salmonella strains was isolated and identified from dead embryo samples collected from 4 hatcheries attached to their parental flocks of Sanhuang Chickens,and the drug resistance and resistance genes were detected by Kirby-Bauer Test and PCR method,respectively.A total of 173 Salmonella isolates were obtained from 356 dead embryos,including 5 serotypes:S.pullorum(162),S.blegdam (5),S.Cerro (4),S.enteritidis(1),S.Fillmore(1).Results showed that the isolation rate and drug resistance of Salmonella in dead embryos from different sources were significantly different.Of total 173 Salmonella isolates,the drug resistance rate to Sulfisoxazole (SF),Ampicillin (AMP),Tetracycline (TE) and Oxytetracycline (OT) were 82.1％,74.0％,49.1％ and 48.6％.And 43.4％ (75/173) isolates showed multiple drug resistance.The resistance to other drugs was mild,and the resistance rate was below 11％.The isolates were sensitive to Ceftazidime (CAZ),Meropenem (MEM),Imipenem (IPM),Polymyxin E (CT),Chloramphenicol (C),Enrofloxacin (ENR) and Ciprofloxacin (CIP).In addition,13 drug-resistant genes were detected by PCR,and the coincidence rate between drug-resistant phenotype and drug-resistant genes was more than 72％,especially the rate were 100％ to extended-spectrum beta-lactamases (ESBLs),aminoglycosides,cephalosporins.The results indicate that serotypes of Salmonella infected in Sanhuang chicken embryos are complex,the multi-drug resistance is serious and the resistance genes are widely existed in these resistant strains.关键词
三黄鸡/死胚/沙门菌/耐药性/耐药基因Key words
Sanhuang chicken/dead embryo/Salmonella/resistance/drug-resistant gene分类
农业科技引用本文复制引用
徐耀辉,邓同炜,齐亚如,韩文龙,卢建洲,蒋增海..三黄种鸡场孵化死胚中沙门菌的分离鉴定与耐药性分析[J].畜牧兽医学报,2018,49(5):1081-1088,8.基金项目
河南省科技攻关计划(162102110144) （162102110144）
江苏省人兽共患病学重点实验室开放课题(R1407) （R1407）
河南牧业经济学院预防兽医学重点学科项目(MXK2016102) （MXK2016102）
