西南林业大学学报2018，Vol.38Issue(3)：21-30,10.DOI:10.11929/j.issn.2095-1914.2018.03.004
樱亚属部分园艺品种遗传多样性的ISSR分析
Genetic Diversity Analysis of Cerasus Cultivars Based on ISSR Markers
摘要
Abstract
In order to analyze relationship and genetic diversity among cultivars of Cerasus, 15 polymorphic primers were screened from 100 primers for genetic analysis of 208 cultivars using ISSR(inter simple sequence re-peats) molecular marker technique. The results showed that a total of 91 clear bands were amplified by 15 primers, in which 83 banks were polymorphic.The average percentage of ISSR polymorphic bands was 91.21%.At the culti-var level,the percentage of polymorphic loci(PPB) was 94.03%,the Nei′s gene diversity index(H) was 0.309 5, and the Shannon′s information index (I) was 0.466 7. At the population level,the average PPB was 55.65%,H was 0.199 9,I was 0.298 0. The genetic parameters of Zhejiang population were the largest,while the Fujian were the smallest. The total gene diversity(Ht) was 0.310 7,and the gene differentiation index(Gst) was 0.356 6. The result of the Nei′s genetic distances was in agreement with that of the genetic similarity coefficients according to UP-GMA (unweighted pair-group method with arithmetic means) analysis. The 208 cultivars could be divided into 2 categories. One category was cultivars from Fujian,Shandong and Yunnan Province,the other from Guizhou,Hubei, Zhejiang and Shanghai Province. The greater genetic diversity in Cerasus cultivars, and most variation was consist-ently originated from the interior of populations,while the cultivars distributions were obviously district specific.关键词
樱亚属/品种/ISSR/遗传多样性Key words
Cerasus/cultivar/ISSR/genetic diversity分类
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蒋冬月,沈凤强,徐卢雨,李因刚,沈鑫,柳新红..樱亚属部分园艺品种遗传多样性的ISSR分析[J].西南林业大学学报,2018,38(3):21-30,10.基金项目
浙江省属科研院所扶持专项(2017F30017,2016F50024)资助 （2017F30017,2016F50024）
浙江省十三五林木新品种选育项目(2016C02056-12)资助. （2016C02056-12）
