新医学2018，Vol.49Issue(5)：309-314,6.DOI:10.3969/j.issn.0253-9802.2018.05.003
CCR5第一和第二胞外环特异性结合短肽对大鼠结肠炎的炎症细胞浸润及NF-κB/TNF-α信号通路的影响
Effects of antagonistic peptides specifically binding to the first and second extracellular loops of CCR5 on inflammatory cell infiltration and NF-κB/TNF-αsignaling pathway in rats colitis
摘要
Abstract
Objective To investigate the effect of the specific binding of antagonistic peptides to the first and second extracellular loops of CC chemokine receptor 5(CCR5)upon the inflammatory cell infiltration and NF-κB/TNF-αsignaling pathway in SD rats with colitis induced by trinitrobenzene sulfonic acid(TNBS). Methods The experimental SD rat models with colitis were induced by 5%TNBS,and intervened by two an-tagonistic peptides of CCR5. Pathological cytological analysis,western blot and PCR were performed to examine the histological changes of colon,proteins and mRNA levels of the NF-κB/TNF-αsignaling pathway. Results Compared with model group,the histological injuries of colitis were alleviated after administration of two antago-nistic peptides of CCR5(both P<0.05). The infiltration of neutrophils,lymphocytes and macrophages in rats treated with GH and HY peptides was significantly reduced(all P<0.05). Besides,the expression levels of the proteins and mRNAs related to the NF-κB/TNF-αsignaling pathway were also significantly down-regulated (all P<0.05). Conclusions Antagonistic peptides specifically binding to the ECL1 and ECL2 of CCR5 can evidently inhibit the inflammation in SD rats with TNBS-induced colitis,probably by suppressing the activation of the NF-κB/TNF-αsignaling pathway.关键词
CC趋化因子受体5/拮抗肽/炎症性肠病/核转录因子-κB/肿瘤坏死因子-αKey words
C-C chemokine receptor 5/Antagonistic peptide/Inflammatory bowel disease/Nuclear factor-κB/Tumor necrosis factor-α引用本文复制引用
宋杨达,刘思雪,宋铱航,沈溪明,黄花荣,钟英强..CCR5第一和第二胞外环特异性结合短肽对大鼠结肠炎的炎症细胞浸润及NF-κB/TNF-α信号通路的影响[J].新医学,2018,49(5):309-314,6.基金项目
国家自然科学基金资助项目(81370499) （81370499）
广东省自然科学基金资助项目(2014A030313020) （2014A030313020）
广州市科技计划项目(20180301004) （20180301004）
