中国免疫学杂志2018，Vol.34Issue(5)：727-731,5.DOI:10.3969/j.issn.1000-484X.2018.05.017
Sel1L对小鼠骨髓源树突状细胞的影响
Effects of Sel1L on properties of bone marrow derived dendritic cells
摘要
Abstract
Objective:To explore the effects of suppress or enhancer of lin-12-like(Sel1L) on differentiation and function of bone marrow-derived dendritic cells.Methods:To generate conditional knockout mice by the Cre-Loxp recombination system.ELISA and Real-time fluorescence quantitative PCR(RT-PCR) was used for analyzing the protein levels and mRNA levels of IL-6/IL-12 in BMDCs.The protein levels of Sel1L in BMDCs were detected by Western bolt.The expression of CFSE,CD80,CD86,MHC-Ⅰ,MHC-Ⅱon BMDCs and the capability in priming OVA specific CD4+T cells proliferation were analyzed by the flow cytometry.Results:The deficiency of Sel1L decreases the proliferation of DCs during its differentiation,up-regulates the secretion of IL-6,IL-12 and the expression of MHC-Ⅰ.Notably,Sel1L-null DCs was failed to up-regulate MHC-Ⅱexpression and dramatically impaired their ability to prime OVA323-339specific CD4+T cell.Conclusion:The deletion of Sel1L can reduce the proliferation of BMDCs and down-regulate its ability in priming the proliferation of OVA specific CD4+T cells.关键词
Sel1L/树突状细胞/内质网应激/共刺激分子/CD4+T细胞Key words
Sel1L/Dendritic cell/Endoplasmic reticulum stress/Co-stimulatory molecule/CD4+T cell分类
医药卫生引用本文复制引用
许洁,龙乔明,夏圣,颜楠楠,赵传祥,李慈,吴仪,肖腾飞,高凤威,周文慧,邵启祥..Sel1L对小鼠骨髓源树突状细胞的影响[J].中国免疫学杂志,2018,34(5):727-731,5.基金项目
国家自然科学基金(31570879,31428006)和江苏省社会发展重点项目(BE2017696)资助. （31570879,31428006）
