中国畜牧兽医2018，Vol.45Issue(5)：1127-1136,10.DOI:10.16431/j.cnki.1671-7236.2018.05.001
水牛溶血磷脂酸受体3基因的克隆及生物信息学分析
Cloning and Bioinformatics Analysis of LPAR3 Gene in Buffalo
摘要
Abstract
The study was aimed to clone and analyze the lysophosphatidic acid receptor 3 (LPAR3)gene of buffalo with bioinformatics method.The ovary was collected from buffalo,DNA samples was extracted and the primers were designed according to the sequence of the buffalo L PA R3 gene(GenBank accession No.:XM_006047539.1).And L PA R3 gene was cloned and analyzed by PCR amplification and bioinformatics softwares.The results showed that L PA R3 gene was 1 552 bp in length containing a CDS sequence of 1 062 bp that encoded 353 amino acids. Sequence homology analysis indicated that the buffalo LPAR3 showed 99.4%,98.9%,98.0%, 88.6%,90.0%,90.3% and 91.2% identity with that of Bison bison,Bos taurus,Ovis aries,Sus scrof a,Equus caballus,Rousettus and Cercocebus atys,which was consistent with phylogenetic tree analysis.The formula of the LPAR3 protein was C1853H2878N476O486S31,and the molecular weight,theory isoelectric point,instability index and grand average of hydrophobicity was 40.59 ku,9.52,47.05 and 0.324,respectively,indicating that it was an alkaline unstable hydro-phobic protein.There were 7 transmembrane domain but no signal peptide which showed it was a non-secretory protein.The secondary structure analysis showed that the percentage of α-helices, random coil and extended strand was 48.16%,41.36% and 10.48%,respectively.Protein subcel-lular localization analysis found that LPAR3 distributed in the plasma membrane(56.5%),endo-plasmic reticulum(26.1%),vacuole(8.7%),Golgi apparatus(4.3%)and the nucleus(4.3%), and might play a role in the transport and combination,purines and pyrimidines of transporters and signal transduction.Thus,those results would lay a foundation for clarifying the mechanism of the gene performance in buffaloes.关键词
水牛/溶血磷脂酸受体3/基因克隆/生物信息学Key words
buffalo/lysophosphatidic acid receptor 3(LPAR3)/gene cloning/bioinformatics分类
生物科学引用本文复制引用
庞春英,梁莎莎,马小娅,邓廷贤,陆杏蓉,段安琴,黄韵琪,梁贤威..水牛溶血磷脂酸受体3基因的克隆及生物信息学分析[J].中国畜牧兽医,2018,45(5):1127-1136,10.基金项目
广西科技攻关项目(桂科合15104001-3) （桂科合15104001-3）
广西科技重大专项(桂科AA16450002) （桂科AA16450002）
