中国畜牧兽医2018，Vol.45Issue(5)：1137-1144,8.DOI:10.16431/j.cnki.1671-7236.2018.05.002
香猪卵巢StAR和CYP11A1基因的差异表达研究
Study on Differential Expression of StAR and CYP11A1 Genes in Xiang Pig Ovary
摘要
Abstract
In order to reveal the molecular mechanism of Xiang pig reproduction,StA R and CY P11A1 genes of ovary in Xiang pigs were analyzed.Chosing two groups of Xiang pig with high and low litter sizes as samples,the expression level of two genes were further detected from ovary by Real-time PCR method.And the promoter regions of two genes were cloned and sequenced from the genomic DNA.The results showed that both of FPKM values from the RNA-seq for transcriptomes and the mRNA levels by Real-time PCR in the group with high litter size were much higher than the other one.In the promoter region of StA R gene with 1 144 bp,the binding elements of a total of 72 transcription factors(TF,34 kinds)were deduced,including ER,COUP and Sp1,etc.About 8 SNP sites were determined from sequence of 68 to 698 bp upstream of the transcription initiation site.All of SNP sites were outside of the binding sites for TFs.For the promoter of CY P11A1 gene in 970 bp,there presented 75 TFs(30 kinds),such as Sp1,PU.1 and C/EBPdel.About 5 SNP sites were detected in the region of 12 to 593 bp upstream of the start site,in which a polymorphism site of C-T at 69 bp would change the binding of SP1.The frequen-cy of T allele was 17% and 33% in Xiang pigs with high or low litter sizes,respectively.The SNP site at 69 bp in the promoter region might be a reason for the different expression of CY P11A1 gene in pig ovary.关键词
香猪/卵巢/实时荧光定量PCR/StAR基因/CYP11A1基因/启动子Key words
Xiang pig/ovary/Real-time PCR/StARgene/CYP11A1gene/promoter分类
农业科技引用本文复制引用
毛宁,王嘉福,张福平,唐靓婷,阮亦麒,易凡利,冉雪琴..香猪卵巢StAR和CYP11A1基因的差异表达研究[J].中国畜牧兽医,2018,45(5):1137-1144,8.基金项目
国家高技术研究发展计划(863计划)课题(2013AA102503) （863计划）
国家自然科学基金(31672390) （31672390）
贵州省农业攻关项目(黔科合支持[2017]2585、[2017]2587) （黔科合支持[2017]2585、[2017]2587）
贵州省"百"层次创新型人才项目(黔科合人才2016-4012号) （黔科合人才2016-4012号）
