中国畜牧兽医2018，Vol.45Issue(5)：1153-1162,10.DOI:10.16431/j.cnki.1671-7236.2018.05.004
乏情和发情初产母猪卵巢microRNA差异表达分析
Analysis on Differentially Expressed microRNA in Ovaries of Anestrous and Estrous Primiparous Sows
摘要
Abstract
To explore the role of ovarian microRNA(miRNA)in regulating the reproductive process in primiparous sows,the miRNA expression profiles were examined using Illumina high-throughout sequencing.The miRNA with high expression and significant difference were analyzed by bioinformatics methods.The results showed that a total of 503 miRNAs were detected in two small RNA libraries,which included 303 known and 200 novel miRNAs.The 3 most highly ex-pressed miRNAs in known miRNAs were ssc-miR-10b,ssc-miR-143-3p and ssc-miR-26a in two small RNA libraries.The highest specific expressed miRNAs in novel miRNAs was chr13_2637_mature,followed by chr8_9994_mature.Compared with estrous primiparous sows,a total of 145 differentially expressed miRNAs(read counts>10,|log2(fold-change)|>1),including 114 up-regulated miRNAs and 31 downregulated miRNAs were identified.31 differentially expressed miRNAs(read counts>1 000,∣log2(fold-change)∣>1)were further screened.In these miR-NAs,chr13_2585_mature was the highest-upregulated,and was discovered only in the ovary of anestrous primiparous sows.Furthermore,7 388 target genes were predicted from 31 miRNAs. KEGG pathway analyses showed that a total of 2 788 target genes were annotated to 297 path-ways,and the top 20 most significantly enriched pathways were involved in reproductive proces-ses,suggesting the 31 miRNAs played a crucial role in the reproductive process of primiparous sows.These results enriched the porcine miRNA database and provided useful information for further research about the reproductive performance of primiparous sow s.关键词
miRNAs/乏情/发情/初产母猪/卵巢Key words
miRNAs/anestrus/estrus/primiparous sow/ovary分类
农业科技引用本文复制引用
任巧玲,白献晓,王璟,郭红霞,陈俊峰,马文涛,和小娥,张家庆,邢宝松..乏情和发情初产母猪卵巢microRNA差异表达分析[J].中国畜牧兽医,2018,45(5):1153-1162,10.基金项目
河南省科技攻关计划项目(162102110036) （162102110036）
河南省农业科学院优秀青年科技基金(2016YQ19) （2016YQ19）
