中国畜牧兽医2018，Vol.45Issue(5)：1163-1169,7.DOI:10.16431/j.cnki.1671-7236.2018.05.005
猪TLR5基因表达水平与F18大肠杆菌侵染关系分析
Analysis of the Relationship Between Expression Level of Porcine TLR5 Gene and Infection of Escherichia coli F18
摘要
Abstract
In order to explore the relationship between Toll-like receptor 5(TLR5)gene expres-sion and resistance to E.coli F18,the bacterial stimulation experiments were conducted in this study.Intestinal epithelial cells(IPEC-J2)were induced by lipopolysaccharide(LPS)and E.coli F18 with different serotypes(F18ab and F18ac),the mRNA expression level of TLR5 gene was detected by Real-time PCR,and the protein expression was detected by Western blotting.The re-sults showed that the expression level of TLR5 gene was extremely significantly up-regulated (P<0.01)after IPEC-J2 cells were infected with different serotypes of E.coli(F18ab and F18ac),which was also extremely significantly up-regulated after IPEC-J2 cells were induced by LPS for 4 and 8 h(P<0.01).After IPEC-J2 cells were induced by LPS for 8 h,the expression level of TLR5 gene increased more significantly than induction for 4 h.After LPS and bacterial stimulation,the expression level of TLR5 protein in cells was extremely significantly up-regulated (P<0.01),which was consistent with the results of mRNA expression.This study further con-firmed that the expression level of porcine T L R5 gene played an important role in the process of cell resistance to E.coli F18 infection by studying the relationship between the expression level of T L R5 gene and E.coli.It also laid the foundation for future research on T L R5 gene function and its application in genetic breeding of E.coli diarrhea.关键词
猪/TLR5基因/大肠杆菌/mRNA表达/WesternblottingKey words
porcine/TLR5 gene/E.coli/mRNA expression/Western blotting分类
农业科技引用本文复制引用
赵呈祥,戴开宇,杜雨露,包文斌,吴圣龙..猪TLR5基因表达水平与F18大肠杆菌侵染关系分析[J].中国畜牧兽医,2018,45(5):1163-1169,7.基金项目
国家自然科学基金(31772560) （31772560）
江苏省重点研发计划(现代农业)(BE2016305) （现代农业）
江苏省研究生科研创新计划项目(XKYCX17_062) （XKYCX17_062）
