中国畜牧兽医2018，Vol.45Issue(5)：1283-1288,6.DOI:10.16431/j.cnki.1671-7236.2018.05.020
利用DNA条形码对海南特种野猪的鉴定研究
Study on Identification of Hainan Special Wild Boar by DNA Barcoding
摘要
Abstract
This study was aimed to explore the feasibility and effectiveness of using a specific seg-ment of pig cytochrome C oxidase subunit Ⅰ(COXⅠ)gene as DNA barcode to identify Hainan special wild boar and other species.COXⅠgene was detected in Hainan special wild boar,Hainan wild boar,Tunchang pig,Wuzhishan pig and Landrace pig,the COXⅠ gene sequences of 13 repre-sentative native pig breeds were downloaded from NCBI,the genetic diversity and genetic distance were analyzed between Hainan special wild boar and other species,and phylogenetic tree was con-structed.The results showed that the sequence of pig COXⅠgene was 1 419 bp,and 67 variation sites of COXⅠ gene were detected in 45 individuals of 5 pig breeds,accounting for 4.7% of the analysis sites,and which 22 sites were parsimony information sites.The intraspecies genetic dis-tance of 5 pig breeds was 0.001 to 0.019,the highest intraspecific genetic diversity was 0.019 of Hainan special wild boar.The interspecific genetic distance of 5 pig breeds was 0.009 to 0.123, the genetic distance between Hainan special wild boar and Landrace pig was the largest(0.123). There was obvious hybrid in Hainan special wild boar,which had clustered with Hainan native breed(Wuzhishan pigs,Tunchang pigs and Hainan wild boar).Hainan special wild boar was loca-ted in a branch with Wuyi Black pigs,Sandu Black pigs,Luchuan pig,the other part of the cluster was located in a branch with Dahuabai pig,and there was no cluster with Landrace pig.The re-sults would provide references for the further breeding of Hainan special wild boar.关键词
海南特种野猪/DNA条形码/鉴定Key words
Hainan special wild boar/DNA barcode/identification分类
农业科技引用本文复制引用
蔡克奇,管庆丰,彭文川,吴科榜..利用DNA条形码对海南特种野猪的鉴定研究[J].中国畜牧兽医,2018,45(5):1283-1288,6.基金项目
海南特种野猪肉质关键基因筛选及分子辅助育种研究及应用(ZDYF2017059) （ZDYF2017059）
海南澄山山猪原种野猪繁育与生态驯养技术研究和应用推广(HD-KYH-2014089) （HD-KYH-2014089）
海南省2016年度研究生创新科研课题(Hys2016-39) （Hys2016-39）
