中国畜牧兽医2018，Vol.45Issue(5)：1312-1318,7.DOI:10.16431/j.cnki.1671-7236.2018.05.024
杜寒绵羊多胎性能候选基因BMPR-1B和BMP15的研究
Study of BMPR-1 B and BMP15 as Candidate Genes for Prolificacy of Duhan Sheep
摘要
Abstract
In order to investigate the mechanism of prolificacy in Duhan sheep at molecular level, the ear tissues of 87 ewes were collected from multiparous Duhan ewes in Shanxi province.The BM P15 and BM PR-1B genes were selected as candidate genes,and its relationship with prolifica-cy,which was associated with the litter size and the primary weight of lambs,was analyzed by the methods of PCR-SSCP and PCR-RFLP.The results showed that A →G mutation occurred in Du-han sheep at 746 bp of the coding region of BM PR-1B gene,at the same time three genotypes were detected:AA,AG and GG,the genotype frequency of AG was 0.5172,higher than that of GG(0.2184)and AA(0.2644),the allele frequency of A(0.5230)was slightly higher than G(0.4770)with A and AG was the dominance allele and genotype,respectively.And the site was in Hardy-Weinberg equlilbrium in Duhan sheep.There was no mutation occurred at 864 bp and al-so no mutation occurred at V31D and S300G sites in BMP15 gene.The number of lambs of GG and AG genotypes were extremely significantly higher than AA genotype(P<0.01)in this popu-lation,but there was no distinct difference between three genotypes in the primary weight of lamb (P> 0.05).It was concluded that the BM PR-1B gene was a major gene affected reproductive traits in Duhan sheep,w hich could be used as a molecular marker to perform molecular assisted selection(MAS)in Duhan sheep.The influence of BMP15 gene mutation affecting prolific ability could be preliminarily excluded in Duhan sheep.关键词
杜寒绵羊/多胎性能/BMPR-1B基因/BMP15基因Key words
Duhan sheep/fecundity trait/BM PR-1B gene/BM P15 gene分类
农业科技引用本文复制引用
郭慧慧,李俊,罗惠娣,牛晋国..杜寒绵羊多胎性能候选基因BMPR-1B和BMP15的研究[J].中国畜牧兽医,2018,45(5):1312-1318,7.基金项目
山西省农业科学院畜牧兽医研究所项目(xms201507) （xms201507）
