中国畜牧兽医2018，Vol.45Issue(5)：1374-1381,8.DOI:10.16431/j.cnki.1671-7236.2018.05.031
新疆北疆地区猪源粪肠球菌的耐药性分析
Drug Resistance Analysis of Swine Enterococcus f aecalis in Northern Xinjiang
摘要
Abstract
In order to determine the drug resistance of swine Enterococcus f aecalis and the distri-bution of genotypes resistance in Northern Xinjiang,total 49 isolates were evaluated for their sen-sitivity to 8 antimicrobial drugs by Kirby-Baller disc diffusion method,and 9 relative resistance genes were detected by PCR method.The amplified fragments were sequenced and compared with the corresponding gene sequences in GenBank.Susceptibility test results showed that the isolates had the highest resistance rate to streptomycin,followed by penicillin and erythromycin,which were highly sensitive to nitrofurantoin and ampicillin.PCR detection results showed that the de-tection rate of β-lactam resistance gene tem was the highest(93.88%),followed by the tetracy-cline resistance gene tetM(85.71%),the detection rates of quinolones resistance gene gyrA and parC both were 42.86%,the detection rates of aminoglycoside resistance genes aph(3′)-Ⅲ,aac (6′)/aph2″and ant(6′)-Ⅰ were 36.73%,16.33% and 16.33%,respectively,and no mef A and ermB genes were detected.The results showed that the multidrug resistance of swine Enterococ-cus f aecalis in Northern Xinjiang was very serious,and its resistance phenotype was not consist-ent with the genotype.关键词
粪肠球菌/抗菌药物/耐药基因Key words
Enterococcus faecalis/antibacterials/drug resistance gene分类
农业科技引用本文复制引用
王蒙蒙,张子荣,欧都·吾吐那生,蒲敬伟,袁立岗,齐亚银..新疆北疆地区猪源粪肠球菌的耐药性分析[J].中国畜牧兽医,2018,45(5):1374-1381,8.基金项目
国家自然科学基金项目"致幼畜脑膜炎粪肠球菌穿越血脑屏障的机制初探"(31760737) （31760737）
国家自然科学基金项目"毒力因子参与不同源肠球菌的机会感染机制及整合子介导的耐药机制初探"(31260610) （31260610）
兵团现代农业科技攻关与成果转化计划项目"生猪健康养殖及安全猪肉生产关键技术集成与示范"(2015AC026) （2015AC026）
