中国畜牧兽医2018，Vol.45Issue(5)：1395-1400,6.DOI:10.16431/j.cnki.1671-7236.2018.05.034
醋酸诱导犬急性溃疡性结肠炎模型的建立
Establishment of Acute Ulcerative Colitis Model Induced by Acetic Acid in Dog
摘要
Abstract
The study was aimed to establish a stable model of acute ulcerative colitis(UC)in dogs using acetic acid.Sixteen healthy dogs were randomly divided into four groups:Control group and three acetic acid(with different concentrations)groups.After anesthetic Quanmianbao injection, the acetic acid groups was perfused with 4%,7% and 10% 2 mL/kg acetic acid,respectively.The control group was not anesthetized and the rectal perfusion was given the same dose of sterile sa-line.The defecation and the colorectal mucosa were observed by electronic endoscopy at the 1st, 3rd and 7th days after injection.All the experimental dogs were sacrificed on the 7th day.The le-sioned tissues were collected and examined by histological.The results showed that:In 4% acetic acid group,short-term dilute feces and mild inflammatory cells infiltration was observed.In 10%acetic acid group,dogs died because of strong inflammation w hich lead to colon perforation.In 7%acetic acid group,both fecal traits and colon endoscopy check showed significant symptoms of co-litis,and ulcer foci formed and droped off after 3 and 7 d of acetic acid administration,respective-ly.Histological examination showed that there was significant inflammatory cells infiltration around the ulcer foci,and it would significantly reduced as time goes on,and replaced by fibroblast hyperplasia.In conclusion,acute ulcerative colitis in dogs could be successfully induced by 7%acetic acid perfusion.关键词
醋酸/犬/溃疡性结肠炎Key words
acetic acid/dog/ulcerative colitis(UC)分类
农业科技引用本文复制引用
陈丽,徐在品,张成桂,巫秀美,许玉兰,陈广,杜建斌..醋酸诱导犬急性溃疡性结肠炎模型的建立[J].中国畜牧兽医,2018,45(5):1395-1400,6.基金项目
云南省昆虫生物医药研发重点实验室专项经费(2015DG030) （2015DG030）
