中国畜牧兽医2018，Vol.45Issue(5)：1401-1407,7.DOI:10.16431/j.cnki.1671-7236.2018.05.035
蛋鸡脂肪肝综合征模型的建立
Establishment of Fatty Liver Syndrome Model of Egg-type Chickens
摘要
Abstract
This study was aimed to establish the fatty liver syndrome model of egg-type chickens. 280 five-day-old Hyline egg-type chickens were selected and randomly divided into control group (basic diet group)and model groups(A,B and C groups).From 0 to 10 days of experiment,the model groups was fed with high-fat diet with different ratio,and the routine basal diet was fed from 11 to 20 days.The experimental chicken mental state,appearance and signs,water intake and appetite of chickens were observed and recorded each day.15 chickens were randomly selected from each group on the 0,10th and 20th days of experiment for blood sampling,liver and abdomi-nal fat extraction.The liver coefficient,hepatic lipid ratio,abdominal fat percentage,serum total cholesterol(TC),triglyceride(TG),alanine transaminase(ALT)and aspartate transaminase (AST)levels were determined.The results showed that there was a lot of fat deposits in the ab-dominal cavity and mesentery in three modle groups on the 10th day of the experiment,the indica-tors of liver coefficient,hepatic fat percentage,abdominal fat percentage,serum TC,TG,ALT and AST were accord with the diagnostic criteria of fatty liver syndrome model of chickens.On the 20th day of experiment,the levels of ALT and AST were recovered in model groups A and B,and the other indexes were higher than those in control group,the clinical symptoms and the detection indexes in model group C were accord with the diagnostic criteria of fatty liver syndrome model of chickens.T herefore,continuous feeding of high-fat diet C(74.5% basal diet,6% cholesterol,14%lard,5% sucrose,0.5% propylthiouracil)could successfully establish the fatty liver syndrome model of egg-type chickens.关键词
蛋鸡脂肪肝综合征/模型建立/高脂饲料/肝脂率/腹脂率Key words
fatty liver syndrome in egg-type chicken/model establishment/high fat diet/hepatic fat rate/abdominal fat rate分类
农业科技引用本文复制引用
邢玉娟,赵尉丹,朱浩,秦俊杰,侯晓礁,王秀敏..蛋鸡脂肪肝综合征模型的建立[J].中国畜牧兽医,2018,45(5):1401-1407,7.基金项目
公益性行业(农业)科研专项经费项目:中兽医关键技术研究与示范(201403051_1_5) （农业）
