中国畜牧兽医2018，Vol.45Issue(5)：1408-1416,9.DOI:10.16431/j.cnki.1671-7236.2018.05.036
藏草乌水煎提取液体外杀灭羊虱蝇药效及皮肤毒性试验研究
Study on the Efficacy and Toxicity of Aconitum Extract on Melophagus ovinus in vitro
摘要
Abstract
The study was aimed to determine the efficacy and toxicity of Aconitum extract on Melophagus ovinus and it's skin toxicity,irritation and allergy in vitro,in order to provide a theo-retical basis for the development,utilization and clinical application of Tibetan A conitum.1%,2%,4%,8% and 10% Tibetan Aconitum extract were dropped in plates lined with absorbent pa-per and Melophagus ovinus to observe the killing efficacy on Melophagus ovinus at 1,2,4,8 h,re-spectively.The same dose of 1%,2% Tibetan Aconitum extract was taken to apply evenly in rab-bits intact and damaged skin to detect the skin toxicity,irritation and allergy of Tibetan A conitum extract.The results showed that at 1 h after administration,all drug groups and positive control group showed a kill effect on Melophagus ovinus in the different levels,and at 4 h after adminis-tration,the killing rate of positive control group was 100%,while that was 70% of 1% and 2%Tibetan Aconitum extract groups at 8 h with 2% drug dose showed the better effect.The skin toxicity test showed that rabbits skin had no erythema and edema,the body weight was normal, and the skin irritation test showed that the extract had no irritation to rabbits skin,while the results of skin allergy showed the it had no allergy to rabbits.In conclusion,Tibetan Aconitum extract had the good killing effects on Melophagus ovinus in vitro,and the skin medication was safe.关键词
藏草乌水煎提取液/羊虱蝇/药效学/刺激性/毒性/过敏性Key words
Aconitum extract/Melophagus ovinus/pharmacodynamics/irritation/toxicity/allergy分类
农业科技引用本文复制引用
宋向东,拉巴次旦,郝宝成,胡永浩,班旦,梁剑平,胡毓瑶,吴金措姆,四郎玉珍,袁振杰,格桑卓嘎..藏草乌水煎提取液体外杀灭羊虱蝇药效及皮肤毒性试验研究[J].中国畜牧兽医,2018,45(5):1408-1416,9.基金项目
西藏自治区财政厅项目"藏兽药的研发与示范" （）
