中国畜牧杂志2018，Vol.54Issue(5)：30-33,4.DOI:10.19556/j.0258-7033.2018-05-030
5个繁殖候选基因在香猪卵巢和子宫中的差异表达
Differential Expression of Five Reproductive Candidate Genes in the Ovary and Uterus of Xiang Pig
摘要
Abstract
The aim of this study was to confirm whether the effects of five candidate genes on ovary and uterus function presented,which were screened out from the transcriptome sequencing data of Xiang pig ovary.The five genes included SLPI(secretory leukocyte peptidase inhibitor),INHA(inhibin alpha subunit),SERPINE1(Serpin Family E Member 1),MSMB(microseminoprotein beta) and PTGFR(prostaglandin F receptor).We compared the expression level of these genes in the ovary and uterus of Xiang pig at two stages of estrus and non-estrus via fluorescence quantitative PCR method.The expression levels of SERPINE1 gene were not different between two stages of ovary and uterus.Compared with the non-estrus period,INHA gene was significantly increased in ovary at estrus stage (P＜0.01),the expression levels of the SLPI gene were significantly increased in the ovary (P＜0.05) and extremely significant higher in the uterus (P＜0.01).The mRNA level of MSMB gene decreased in ovary and uterus (P＜0.05),and the expression levels of INHA and PTGFR genes were significantly declined in the uterus during the estrous period of Xiang pig (P＜0.05).Our results implied that INHA,SLPI and MSMB might have roles in both of ovary and uterus,while PTGFR might have a function only on uterus of Xiang pig.关键词
香猪/卵巢/子宫/SLPI/INHA/SERPINE1/MSMB/PTGFRKey words
Xiang pig/Ovary/Uterus/SLPI/INHA/SERPINE 1/MSMB/PTGFR分类
农业科技引用本文复制引用
易凡利,王嘉福,张福平,阮亦麒,唐靓婷,毛宁,冉雪琴..5个繁殖候选基因在香猪卵巢和子宫中的差异表达[J].中国畜牧杂志,2018,54(5):30-33,4.基金项目
国家高技术研究发展计划(863计划)课题(2013-AA102503) （863计划）
国家自然科学基金(31672390) （31672390）
贵州省“百”层次创新型人才项目(黔科合人才2016-4012号) （黔科合人才2016-4012号）
贵州省农业攻关项目(黔科合支撑[2017]2585、[2017]2587,黔科合NY[2013]3073号) （黔科合支撑[2017]2585、[2017]2587,黔科合NY[2013]3073号）
