中国畜牧杂志2018，Vol.54Issue(5)：58-61,4.DOI:10.19556/j.0258-7033.2018-05-058
初情期启动过程中小尾寒羊下丘脑GnRH基因甲基化状态及表达量关系研究
Relationship between Methylation Status and Expression of GnRH Gene in Small Tail Han Sheep during Onset of Puberty
摘要
Abstract
Early or late onset of puberty concems the reproductive performance of females.GnRH is the key gene during the onset of puberty,and the relationship between the methylation status of promoter and mRNA expression is unclear.In this paper,the hypothalamus of Small Tail Han Sheep in peri-puberty,pre-puberty and puberty were selected as samples,bisulfite sequencing PCR (BSP) and realtime fluorescence quantitative PCR(qRT-PCR) was used to study the methylation status and GnRH mRNA expression in GnRH promoter region of Small Tail Han Sheep in different stages of puberty.The results showed that with the onset of puberty,the methylation level of GnRH gene promoter significantly decreased,especially in the promoter region-570;meanwhile,with the onset of puberty the expression of GnRH mRNA significantly (P＜0.05)increased.The results suggest that during the onset of puberty,the increased expression of GnRH mRNA may be related to the decrease of methylation level at specific site in the promoter region of GnRH gene in hypothalamic.关键词
初情期/下丘脑/促性腺激素释放激素/甲基化Key words
Puberty/Hypothalamus/Gonadotropin-releasing hormone/Methylation分类
农业科技引用本文复制引用
丁赫,宫永胜,王军,吕文发..初情期启动过程中小尾寒羊下丘脑GnRH基因甲基化状态及表达量关系研究[J].中国畜牧杂志,2018,54(5):58-61,4.基金项目
国家自然科学基金(31402073) （31402073）
吉林省教育厅科学技术研究项目(JJKH20170319KJ) （JJKH20170319KJ）
