中国畜牧杂志2018，Vol.54Issue(5)：62-66,5.DOI:10.19556/j.0258-7033.2018-05-062
体外培养鸽嗉囊组织形态学、酶活力及基因表达动态变化规律
Dynamic Changes of Histological Structure, Enzymes Activities and Genes Expressions of Pigeon Crop in Tissue Culture in vitro
摘要
Abstract
This study was conducted to investigate dynamic changes of histological structure,enzymes activities and genes expressions of pigeon crop in tissue culture in vitro.The crop from health White King meat-type pigeons (at the age of 60) were collected and cultivated for 7 days in vitro.Histological structure,activities of metabolism-and apoptosis-related enzymes and expressions of cytokertin-and apoptosis-related genes of pigeon crop were analyzed in tissue culture in vitro.The histological assessment indicated that structure of crop were integrated,cells in the epithelial layer lined up densely from 2 to 4 days after culture in vitro,then the epithelial structures of crop were severely degenerated after 5 days culture in vitro.The results of enzymeological assay and genes expressions showed that activity of Caspase-3 on the 1 st,5th,6th and 7th day after culture was significantly higher than that on the 2nd,3rd and 4th day after culture in vitro (P＜0.05).Activities of succinate dehydrogenase,Na+-K+-ATPase,Ca2+-Mg2+-ATPase and total ATP aseand genes expressions of Bcl-2 and cytokertin-19 were significantly reduced on the 1 st,6th and 7th day of culture in vitro,and increased from 2 to 4 days after culture in vitro (P＜0.05).Bak1 gene expression remained stable from 2 to 5 days after culture in vitro,then it was highly up-regulated again on the 6th day after culture in vitro (P＜0.05).The results indicated that the histological structure,enzymes activities and genes expressions of pigeon crop changed significantly in tissue culture in vitro with the incubation going on,and the optimal culture time was 4 days.Additionally,pigeon crop tissueshould bepre-cultured in vitro forld before other researches of pharmacology,pathology and physiology.关键词
鸽嗉囊/组织体外培养/形态学/酶活力/基因表达Key words
Pigeon crop/Tissue culture in vitro/Histological structure/Enzymes activities/Gene expressions分类
农业科技引用本文复制引用
万雪萍,谢鹏,卜柱,汤青萍,邹晓庭..体外培养鸽嗉囊组织形态学、酶活力及基因表达动态变化规律[J].中国畜牧杂志,2018,54(5):62-66,5.基金项目
国家自然科学基金(31501974) （31501974）
江苏省自然科学基金(BK20150462) （BK20150462）
江苏省重点研发项目(现代农业)(BE2017348) （现代农业）
