中国医学科学院学报2018，Vol.40Issue(1)：7-12,6.DOI:10.3881/j.issn.1000-503X.2018.01.002
不同剂量辐射对NOD/SCID小鼠肠损伤的影响
Effect of Different Doses of Radiation on Intestinal Injury in NOD/SCID Mice
摘要
Abstract
Objective To study the effect of different doses of radiation on intestinal injury,with an attempt to find the optimal radiation dose for establishing intestinal injury models in NOD/SCID mice.Methods Forty healthy male SPF-grade NOD/SCID mice were divided randomly into four groups:blank control group and 4-,5-,6-Gy irradiatio groups,with 10 mice in each group.The irradiation rate was 1 Gy/min.The general conditions,body weight,intestinal bacterial translocation,and histopathologic changes were observed and compared.Results The survival rate was 60％,50％,and30％ inthe4-,5-,and6-Gygroups15 days after irradiation,and the intestinal bacterial translocation rate was 20％,50％,and 70％,respectively.The body weights in 5-Gy group (P =0.015) and 6-Gy group (P =0.011) were significantly higher than that in blank control group.The length of small intestinal villi decreased in the 4-Gy group.In the 5-Gy group,the structure of intestinal mucosa villi became wide,flat,and inverted,along with the shedding of epithelial cells,the atrophy of glands,and the damage of recess structures.In the 6-Gy group,the structure of intestinal mucosal villi was damaged,the villi were ruptured and smashed,and the recess structures were missing;meanwhile,there was a large amount of inflammatory cell infiltration between tissues,along with visible spotty bleeding and necrosis.The length of small intestine villi in the blank control group and 4-,5-,and 6-Gy groups were (361.77 ±22.77),(291.68 ± 32.45),(248.03 ± 51.09),and (195.90 ± 26.39) μm,respectively.In particular,it was significantly shorter in 4-Gy group (P =0.005),5-Gy group (P ＜ 0.001),and 6-Gy (P ＜ 0.001) than in the blank control group,was significantly shorter in the 5-Gy group (P =0.041) and 6-Gy group (P =0.001)than in the 4-Gy group,and significantly shorter in 6-Gy group than in the 5-Gy group (P =0.020).Conclusion 5-Gy irradiation in mice models can decrease body weight,cause the damage of intestinal mucosa and the shedding of inflammatory cells,with stable survival rate and bacterial translocation rate.关键词
辐射/肠损伤/NOD/SCID小鼠/模型Key words
radiation/intestine injury/NOD/SCID mice/model分类
医药卫生引用本文复制引用
杜彬,原杰,任召祺,安媛,江峰,李军,杨超..不同剂量辐射对NOD/SCID小鼠肠损伤的影响[J].中国医学科学院学报,2018,40(1):7-12,6.基金项目
国家自然科学基金(31600681)和全军医学科技青年培育项目(17QNP029) Supported by the National Natural Sciences Foundation of China (31600681) and the Military Medical Science and Technology Youth Training Program (17QNP029) （31600681）
