中国真菌学杂志2018，Vol.13Issue(2)：65-70,6.
SakA对马尔尼菲篮状菌药物应激及致病力的影响
Effects of SakA on the drug stress and virulence in Talaromyces marneffei
摘要
Abstract
Objective Environmental stress signals are transmitted by evolutionarily conserved MAPKs in most fungi,such as Hog1 in the budding yeast Saccharomyces cerevisiae,Spcl in the fission yeast Schizosaccharomyces pombe and SakA in the Aspergillus nidulans.It has been shown that in Talaromyces marneffei,a pathogenic dimorphic fungus,the SakA (HOG1) gene is involved,not only in tolerance against oxidative and heat stresses,but also in playing a role in growth,morphology,asexual development and the integrity of the cell wall.In this study,the role of the TM SakA gene on the drug Stress and virulence were investigated.Methods We constructed a SakA knockout strain (AsakA) of TM to observe the coculture of spores with macrophages and compare the growth status of wild plants and △sakA at drug pressures.In addition,we infected mice with wild and knockout strains,respectively,and the mortality and fungi loading were compared between these two groups.Results After being phagocytosed by macrophages for 24 h,the sausage-like yeast cells were observed in the wild-type strain while the △sakA was still in the form of spores and could not be transformed into yeast cells.The number of colonies formed by △sakA co-cultivation was less than that of the wild-type strain.Compared with the wild strain,△sakA is more sensitive to echinodermycin drugs.In the infected mouse model,the mortality and fungi loading of △sakA-infected mice were significantly lower than those of wild strains.Conclusion SakA gene plays an important role in the pathogenesis of TM and is associated with why the wild strains of TM is not sensitive to echinodermycin.关键词
SakA/马尔尼菲篮状菌/药物应激/致病力Key words
SakA/Talaromyces marneffei/drug stress/virulence分类
医药卫生引用本文复制引用
宁心强,魏金瑛,郑艳青,梁浩,曹存巍..SakA对马尔尼菲篮状菌药物应激及致病力的影响[J].中国真菌学杂志,2018,13(2):65-70,6.基金项目
国家自然科学基金(81571971,81271804) （81571971,81271804）
