中国真菌学杂志2018，Vol.13Issue(2)：71-74,4.
实时荧光定量PCR检测马尔尼菲篮状菌病组织样本中菌载量的研究
The research of quantitative real-time PCR testing different specimen in diagnosis of talaromycosis
摘要
Abstract
Objective To evaluate real-time fluorescence quantitative PCR (Qpcr) in determining the load of Talaromyces marneffei in different samples of talaromycosis marneffei (TSM).Methods The loads of Talaromyces marneffei in pathological slices,biopsy tissues from suspected patients of TSM were measured by means of Qpcr.Results The average load of Talaromyces marneffei in pathological slices was 3.01 × 104 copies (1.07 × 104 ～7.26 × 104 copies),the load of Talaromyces marneffei in biopsy tissues was 4.68× 105 copies.Conclusion This study showed that Qpcr determining the load of Talaromyces marneffei in pathological slices and biopsy tissues could be a useful method in TSM diagnosis.关键词
马尔尼菲篮状菌病/实时荧光定量PCR/分子诊断/病理切片/淋巴结活检组织Key words
talaromycosis marneffei (TSM)/qPCR/diagnosis/pathological slices/biopsy tissues分类
医药卫生引用本文复制引用
郑艳青,史娜娜,曹存巍..实时荧光定量PCR检测马尔尼菲篮状菌病组织样本中菌载量的研究[J].中国真菌学杂志,2018,13(2):71-74,4.基金项目
国家自然科学基金(81571971,81271804) （81571971,81271804）
