中成药2018，Vol.40Issue(5)：1083-1087,5.DOI:10.3969/j.issn.1001-1528.2018.05.015
葛根芩连丸UPLC指纹图谱建立及7种成分测定
Establishment of UPLC fingerprints of Gegen Qinlian Pills and determination of seven constituents
摘要
Abstract
AIM To establish the UPLC fingerprints of Gegen Qinlian Pills (Puerariae lobatae Radix,Scutellariae Radix,Coptidis Rhizoma and Glycyrrhizae Radix et Rhizoma Praeparata cum Melle) and to determine the contents of puerarin,baicalein,palmatine,wogonin,daidzin,daidzein and jateorhizine.METHODS The analysis of 70％ methanol extract of this drug was performed on a 30 ℃ thermostatic Waters ACQUITY UPLC(C) BEH C18column,with the mobile phase comprising of methanol-0.1％ glacial acetic acid flowing at 0.3 mL/min in a gradient manner,and the detection wavelength was set at 260 nm.RESULTS There were twenty-five common peaks in the fingerprints of ten batches of samples with the similarities of more than 0.98.Seven constituents showed good linear relationships within their own ranges (r ＞ 0.999 0),whose average recoveries were 98.99％-103.6％ with the RSDs of 1.13％-2.03％.CONCLUSION This simple,reliable and reproducible method can be used for the quality control of Gegen Qinlian Pills.关键词
葛根芩连丸/指纹图谱/化学成分/UPLCKey words
Gegen Qinlian Pills/fingerprints/chemical constituents/UPLC分类
医药卫生引用本文复制引用
吴亿晗,王迪,杨晓琴,李维,傅超美,章津铭,邹亮..葛根芩连丸UPLC指纹图谱建立及7种成分测定[J].中成药,2018,40(5):1083-1087,5.基金项目
四川省教育厅高校科研创新团队(17TD0010) （17TD0010）
