中成药2018，Vol.40Issue(5)：1093-1096,4.DOI:10.3969/j.issn.1001-1528.2018.05.017
HPLC法同时测定退银合剂中6种成分
Simultaneous determination of six constituents in Tuiyin Mixture by HPLC
摘要
Abstract
AIM To establish an HPLC method for the simultaneous content determination of chlorogenic acid,loganin,paeoniflorin,baicalin,salvianolic acid B and paeonol in Tuiyin Mixture (Moutan Cortex,Paeoniae Radix Rubra,Lonicerae japonicae Caulis,etc.).METHODS The analysis of methanol extract of this drug was performed on a 30 ℃ thermostatic ZORBAX SB-Aq column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1％ phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 235 nm.RESULTS Six constituents showed good linear relationships within their own ranges (r≥0.999 7),whose average recoveries were 97.1％-100.6％ with the RSDs of 1.35％-2.28％.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Tuiyin Mixture.关键词
退银合剂/绿原酸/马钱苷/芍药苷/黄芩苷/丹酚酸B/丹皮酚/HPLCKey words
Tuiyin Mixture/chlorogenic acid/loganin/paeoniflorin/baicalin/salvianolic acid B/paeonol/HPLC分类
医药卫生引用本文复制引用
鲁静,牛晓静,段晓颖..HPLC法同时测定退银合剂中6种成分[J].中成药,2018,40(5):1093-1096,4.基金项目
2014年河南省中医药科学研究专项课题(2014ZY02041) （2014ZY02041）
