中成药2018，Vol.40Issue(5)：1097-1100,4.DOI:10.3969/j.issn.1001-1528.2018.05.018
HPLC法同时测定金兰消炎片中4种成分
Simultaneous determination of four constituents in Jinlan Xiaoyan Tablets by HPLC
摘要
Abstract
AIM To establish an HPLC method for the simultaneous content determination of four constituents in Jinlan Xiaoyan Tablets (Lonicerae japonicae Flos,Paeoniae Radix Rubra,Salviae miltiorrhizae Radix et Rhizoma,etc.).METHODS The analysis of 80％ methanol extract of this drug was performed on a 30 ℃ thermostatic Wondasil C1s column (250 mm ×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1％ phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 327,230,286 and 250 nm.RESULTS Chlorogenic acid,paeoniflorin,salvianolic acid B and glycyrrhizic acid showed good linear relationships within the ranges of 46.48-464.84 μg/mL (r =0.999 5),20.28-202.75 μg/mL (r=0.999 1),12.44-124.35 μg/mL (r =0.999 6) and 15.09-150.89 μg/mL (r =0.999 9),whose average recoveries were 99.30％,99.10％,99.38％ and 100.27％ with the RSDs of 1.35％,1.37％,1.28％ and 0.86％,respectively.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Jinlan Xiaoyan Tablets.关键词
金兰消炎片/绿原酸/芍药苷/丹酚酸B/甘草酸/HPLCKey words
Jinlan Xiaoyan Tablets/chlorogenic acid/paeoniflorin/salvianolic acid B/glycyrrhizic acid/HPLC分类
医药卫生引用本文复制引用
华之卉,刘栋,李明春..HPLC法同时测定金兰消炎片中4种成分[J].中成药,2018,40(5):1097-1100,4.基金项目
军队医疗机构制剂标准提高科研专项课题重点项目(14ZJZ02) （14ZJZ02）
