中山大学学报（自然科学版）2018，Vol.57Issue(3)：120-127,8.DOI:10.13471/j.cnki.acta.snus.2018.03.016
贵州壮族人群25个Y-STR基因座遗传多态性
Genetic polymorphism of 25 Y-STR loci in Zhuang population in Guizhou
摘要
Abstract
To determine polymorphic features of 25 Y chromosome Short Tandem Repeats (Y-STR )lociin the Zhuang ethnic and their differences from the corresponding features in other ethnics in the Guizhouprovince,we analyzed the 25 Y-STR loci in the Zhuang ethnic population to obtained genetic polymorphismof the ethnic group.We amplified 25 Y chromosome Short Tandem Repeats loci from the DNAsamples of 67 people of Zhuang ethnic using an Y27 Plex fluorescence detection kit,followed by electrophoresiswith a Genetic Analyzer.Genotyping results were analyzed with a series of genetic software,includingArlequin 3.5,Mega 7.0,and SPSS19.0.Two double-copy loci DYS385ab and DYS387S1 werefound to have 8,10 alleles,whereas the remaining 23 single-copy loci were found to have 86 differentkinds of alleles with the allele number varying from 2 to 7,the allele frequency ranging from 0.014 9 to0.970 1,and the genetic diversity varying from 0.058 8 (for DYS456)to 0.823 6 (for DYS387S1).Atotal of 36 haplotypes were detected,of which 66.7% were unique.The haplotype diversity was 0.957 9.The Rstgenetic distance matrix showed that the genetic structure of the paternal network of the Zhuangethnic group in Guizhou was similar to that of the Fujian She ethnic and Inner Mongolia Mongolian.Thepolymorphism of the 25 Y-STR loci in Guizhou's Zhuang ethnic group differed significantly from that ofthe Yunnan Hani,Xinjiang Uygur,Liaoning Manchu,Jilin Korean and Salzburg,with the greatest differencefrom that of the Xinjiang Kazak,Amdo Tibetan,and West African people.The 25 Y-STR loci inthe Guizhou's Zhuang ethnic group had great genetic polymorphisms,which can be used in the identificationof parental relationship.关键词
Y染色体短串联重复序列/单倍型/遗传多态性/壮族Key words
Y-STR/haplotype/genetic polymorphism/Zhuang ethnic group分类
基础医学引用本文复制引用
国学红,何燕,张秀秀,张婷,王婵娟,官志忠..贵州壮族人群25个Y-STR基因座遗传多态性[J].中山大学学报（自然科学版）,2018,57(3):120-127,8.基金项目
国家自然科学基金(31560306) （31560306）
贵州省科技厅社会发展攻关项目(SY[2012]3135) （SY[2012]3135）
贵阳市科技计划项目([2017]5-9) （[2017]5-9）
