中山大学学报（医学科学版）2018，Vol.39Issue(3)：335-340,6.
MicroRNA-200c在结直肠癌中的表达及对侵袭和转移的影响
Expression of MicroRNA-200c in Colorectal Carcinomas and Its Role on Tumor Cell Migration and Invasion
摘要
Abstract
[Objective] To investigate the expression of microRNA-200c (miR-200c) in colorectal carcinomas (CRC),and analyze its role on tumor cell migration and invasion.[Methods] The expression levels of miR-200c in CRC tissues and adjacent normal mucosa were assessed by real-time quantitative RT-PCR (qRT-PCR).miR-200c mimics were transiently transfected into human colorectal cancer cells,and their roles on cell migration and invasion were analyzed by Transwell assay.Cell proliferation was measured using the Cell Counting kit-8.The expression levels of epithelial and mesenchymal markers as well as related transcription factor ZEB1 were detected by Western blotting.[Results] Lower miR-200c expression was found in primary CRC tissues with lymph node metastasis compared to those without lymph node metastasis and adjacent normal mucosa.Transfection of miR-200c mimics suppressed proliferation,and reduced invasion and migration in SW620 cells.Furthermore,up-regulation of miR-200c inhibited ZEB1,and resulted in increased E-cadherin and reduced Vimentin gene expression.[Conclusion] miR-200c was associated with invasive and metastatic behavior of CRC.These effects may be mediated through regulation of epithelial-mesenchymal transition.关键词
microRNA-200c/结直肠癌/肿瘤转移/上皮间质转化Key words
microRNA-200c (miR-200c)/colorectal carcinomas/tumor metastasis/epithelial-mesenchymal transition分类
医药卫生引用本文复制引用
叶伟标,徐咏强,李妤玲,卢碧燕,杨湘玲,刘焕亮,李仲均..MicroRNA-200c在结直肠癌中的表达及对侵袭和转移的影响[J].中山大学学报（医学科学版）,2018,39(3):335-340,6.基金项目
国家自然科学基金青年科学基金(81702399) （81702399）
广东省医学科研基金(C2017034) （C2017034）
