中山大学学报（医学科学版）2018，Vol.39Issue(3)：349-355,7.
MiR-192/-215靶向BIVM基因调控胃癌细胞生物学功能
MiR-192/-215 Regulates the Biological Functions of Gastric Cancer Cells by Targeting BIVM
摘要
Abstract
[Objective] To investigate the molecular mechanism of miR-192/-215 targeting BIVM in human gastric cancer.[Methods] First,BIVM was the target gene of miR-192/-215 screened by the target gene prediction in solico and gene microarrays.Real-time quantitative PCR verified the results of the microarrays.Then the double-luciferase reporter plasmids were constructed to test that BIVM was the target gene of miR-192/-215.Subsequently,BIVM-siRNA was transfected,to know the effects of BIVM-siRNA on proliferation and apoptosis of gastric cancer cells.8 nude mice were randomly divided into two groups:BIVM-siRNA experimental group and NSC-siRNA control group.Gastric cancer cells transfected with BIVM-SiRNA were implanted under the skin of nude mice to observe the effect of BIVM on the tumorigenicity of gastric cancer cells.[Results] BIVM was screened as miR-192/-215 target gene by gene microarrays and quantitative PCR.Double-luciferase reporting assays were performed to identify the BIVM as miR-192/-215 target gene.The cell proliferation assays showed that BIVM promoted the proliferation of gastric cancer cells (P＜0.05).Test of flow cytometry showed that BIVM inhibited the apoptosis of gastric cancer cells (P＜0.05).Mouse tumorigenesis test confirmed that BIVM could promote gastric cancer cells growth in vivo (P＜0.05).[Conclusion] BIVM plays a role in the carcinogenesis of gastric cancer,and miR-192/-215 targeting BIVM promotes the development of gastric cancer.关键词
胃癌/miR-192/-215/BIVMKey words
gastric cancer/miR-192/-215/BIVM分类
医药卫生引用本文复制引用
林慧娟,张江宇,杨梦婷,陈文静,李辉斌,王健,张小静..MiR-192/-215靶向BIVM基因调控胃癌细胞生物学功能[J].中山大学学报（医学科学版）,2018,39(3):349-355,7.基金项目
广东省自然科学基金(2017A030313479) （2017A030313479）
