中国现代中药2018，Vol.20Issue(5)：522-528,7.DOI:10.13313/j.issn.1673-4890.20171117001
基于高通量测序的女贞果实转录组研究
Transcriptome Analysis for Ligustrum lucidum Fruit Based on Illumina Sequencing Technology
摘要
Abstract
Objective:This study used Illumina/Solexa Hiseq2000 platform to sequence the transcriptome of Ligus-trum lucidum fruits.Methods:A large number of data obtained by sequencing were spliced and assembled,and bioinformatics methods were used to study the functional annotation,functional classification,metabolic pathway analysis and simple sequence repeat site search.Results:The results showed that 32 856 614 clean reads were acquired including 4.93 Gb valid data.The Trinity software was used to assemble the clean reads,receiving 163 092 unigenes with a total read length of 108.57 Mb,an average read length of 665.68 bp and N50of 1345 bp.The 83 903 unigenes were annotated using BLAST searches against the public database(Nr,Nt and SwissProt database).Further functional classification indicates that 16 876 unigenes were classified into cellular components,molecular functions and biological processes in GO database.Aligning unigenes with COG database,25 functional categories could be obtained,among which lots of unigenes involved in nutritional function of fruit to synthesize and transport substances.As the search object,unigene could be located into 21 kinds of metabolic pathways in KEGG database,in which the number of unigene involved in the production of important secondary metabolites flavonoids and terpenoids was 258 and 929,respectively.The analysis for SSR loci showed that 15 925 SSR loci could be detected from 14 270 unigenes.Conclusion:In this study,the L.lucidum fruits was studied systematically at the transcriptome level,which laid a foundation for the further development of molecular markers and biosynthesis of important secondary metabolites from L.lucidum.关键词
女贞果实/转录组/高通量测序/基因注释/SSR分析Key words
Ligustrum lucidum fruit/transcriptome/high-throughput sequencing/gene annotation/SSR analysis引用本文复制引用
徐德宏,罗月芳,江灵敏,孟蕾,谭朝阳..基于高通量测序的女贞果实转录组研究[J].中国现代中药,2018,20(5):522-528,7.基金项目
湖南省教育厅科研项目(15C1040) （15C1040）
湖南省"中药学"重点学科建设项目(湘教通[2011]76号,zy201506,zy201507) （湘教通[2011]76号,zy201506,zy201507）
