中国现代中药2018，Vol.20Issue(5)：593-598,6.DOI:10.13313/j.issn.1673-4890.20171010003
野蔷薇根总黄酮的提取及纯化工艺
Extraction and Purification of Total Flavonoids from Rosa multiflora
摘要
Abstract
Objective:To optimize the extraction process and purification process of the total flavonoids from Rosa multiflora.Methods:The best combination of ethanol concentration,the ratio of material to liquid and extraction time was de-termined by L9(34)orthogonal experiment.The best model of the macroporous adsorption resin for purification was screened out with the method of static adsorption-desorb and dynamic adsorption-desorb,and also the best purifing conditions were made sure by investigating the single factor.Results:Under the UV 508nm detection wavelength,the best extractive combination was to reflux extract total flavonoids with 50% ethanol solution,the ratio of material to liquid 1:10 under 90 ℃,90 min.In 14 types of macroporous resin,HPD-400 was the best one in adsorption-desorb capacity.The best sample concentration was 1.5 times diluted with mother liquid,sample volume was 35 mL and the flow rate was 3 s/drop.The water volume for purifica-tion was 40 mL and the flow rate was 1 s/drop;50% ethanol solution for elution was 32 mL and the flow rate was 2 s/drop. Conclusion:Under the optimal conditions of extraction and purification,the content of the total flavonoid can reach above 50.00%.关键词
野蔷薇根/总黄酮/大孔吸附树脂/提取/纯化Key words
Rosa multiflora Thunb./total flavonoids/macroporous resin/extraction/purification引用本文复制引用
李开言,张飞,田萍,马开,王艳艳,李鸣,王军..野蔷薇根总黄酮的提取及纯化工艺[J].中国现代中药,2018,20(5):593-598,6.基金项目
河南省中医药研究院公益预研资金项目(1603582) （1603582）
国家中医药管理局重点学科建设项目(国中医药发[2009]30号) （国中医药发[2009]30号）
河南省中医药科学研究专项重点课题(2014ZY01021) （2014ZY01021）
河南省中医临床学科领军人才计划(2013-10) （2013-10）
