郑州大学学报（理学版）2018，Vol.50Issue(2)：106-110,5.DOI:10.13705/j.issn.1671-6841.2017088
小鼠CD47-Fc融合蛋白表达载体构建、表达纯化及其生物学活性研究
Expression, Purification and Characterization of Mouse CD47-Fc Fusion Protein in Mammal Cells
摘要
Abstract
By using the eukaryotic expression vector pcDNA 3.1(-),recombinant proteins of CD47-Fc were expressed in the eukaryotic cells of 293T.Purified by affinity chromatography,high purity of CD47-Fc proteins was obtained.The activities of the recombinant protein were evaluated by immunofluorescence staining,flow cytometry, and cell adhesion assay.The results showed that CD47-Fc proteins bound to SIRPαexpressed on the surface of peritoneal macrophage cells.A fusion protein of CD47-Fc which was biological active,was successfully expressed in the eukaryotic cells of 293T.关键词
CD47/SIRPα/亲和层析/重组蛋白Key words
CD47/SIRPα/affinity chromatography/recombinant protein分类
生物科学引用本文复制引用
郭亚楠,陈阳,崔利董,赵梅杰,张守涛..小鼠CD47-Fc融合蛋白表达载体构建、表达纯化及其生物学活性研究[J].郑州大学学报（理学版）,2018,50(2):106-110,5.基金项目
国家自然科学基金青年科学基金项目(31501094) （31501094）
河南省基础与前沿技术研究项目(152300410032). （152300410032）
