安徽农业科学2018，Vol.46Issue(10)：92-93,122,3.
变异冬红果无菌体系的建立
The Establishment of Sterile System of Mutant Malus spectabilis
摘要
Abstract
[Objective] To establish the sterile system for the first generation of mutant Malus spectabilis.[Method] With mutant Malus spectabilis stem section as explant,three disinfection methods were adopted.By comparing the pollution rate,the mortality of browning and the survival rate,the suitable method of disinfection was selected.[Result] The optimal disinfection method with 0.05％ mercuric chloride solution was that the mercuric chloride solution was sterilized for 20 min,the contamination rate was 17.78％,the mortality rate of browning was 11.11％,the survival rate was 71.11％.The optimal disinfection method for mercury solution was disinfection of mercuric chloride solution for 8 min,the contamination rate was 22.22％,browning mortality rate was 20.00％,survival rate was 50.00％.The best disinfection method with 75％ alcohol and 0.1％ mercuric chloride solution was 75％ alcohol for 30 s and 0.1％ mercuric chloride solution for 10min,the contamination rate was 5.56％,the browning mortality rate was 55.56％ and the survival rate was 38.89％.[Conclusion] The best method was 0.05％ mercuric chloride solution disinfection for 20 min,the pollution rate was 17.78％,browning mortality was 11.11％,survival rate was 71.11％.关键词
变异冬红果/消毒处理/组织培养Key words
Mutant Malus spectabilis/Disinfection treatment/Tissue culture分类
农业科技引用本文复制引用
韦晨,王醒..变异冬红果无菌体系的建立[J].安徽农业科学,2018,46(10):92-93,122,3.基金项目
镇江市重点研发计划-现代农业(NY2015013) （NY2015013）
江苏省农业科技自主创新资金项目[CX(15)1004]. （15）
